Refining the seminal biomarker detection: metabolome profiles before and after the liquefaction procedure

ABSTRACT: Semen metabolome analysis presents a promising approach to identify potential biomarkers. However, it remains unclear whether metabolites should be analyzed immediately from fresh ejaculate or after the liquefaction process in conjunction with routine semen quality assessments. To address this question, we set out to evaluate metabolome profiles in semen before and after liquefaction using untargeted metabolomics, with the goal of establishing a protocol for biomarker discovery. Thirty semen samples were collected from 15 healthy donors before and after the liquefaction process and a total of 1664 metabolites were detected in two different analytical approaches. The semen liquefaction process does not affect the overall metabolic profile.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS11207 | MetaboLights | 2025-04-02

REPOSITORIES: MetaboLights