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Ectopic intestinal colonization by an oral commensal promotes C. difficile infection in Crohn’s disease

ABSTRACT: Crohn’s disease is a severe inflammatory disorder of the intestine for which there is no cure. People suffering from Crohn’s disease are at increased risk of developing Clostridioides difficile infection (CDI), which considerably exacerbates symptoms. Using a prospective observational clinical study combined with animal models of intestinal inflammation, we show that ectopic colonization of the intestine by Veillonella, an oral commensal, promotes CDI in Crohn’s disease. We discovered that Veillonella suppresses expression of the main transporter facilitating bile acid reabsorption, which leads to increased bile acid-dependent triggering of C. difficile germination. Our results further indicate that high pro-inflammatory capacity of its lipopolysaccharide render Veillonella exceptionally potent to trigger that mechanism. These findings highlight the potential of otherwise innocuous oral commensals to exacerbate intestinal disease and will assist in the design of therapeutics to treat CDI as a main complication of Crohn’s disease patients.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12275 | MetaboLights | 2025-08-14

REPOSITORIES: MetaboLights

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			Action	DRS
	CD10_NEG.mzXML	Mzxml
	CD12_NEG.mzXML	Mzxml
	CD14_NEG.mzXML	Mzxml
	CD16_NEG.mzXML	Mzxml
	CD18_NEG.mzXML	Mzxml

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