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Identification of Biomarkers using Metabolomics in Porcine Hemorrhagic Shock

ABSTRACT: Hemorrhagic shock with injury results in alterations of the metabolic state of an organism, which contribute to organ dysfunction and death. Previous investigations have explored the effects of carbohydrate prefeed in murine models but few in clinically relevant large animal models. We performed carbohydrate prefeed in pigs undergoing simulated polytrauma and hemorrhagic shock with resuscitation to determine if carbohydrate prefeeding if the metabolic response to shock is dependent on fed state. Sixty-four Yorkshire pigs were divided into two experimental groups: fasted and prefed in additon to two Control groups. Experimental animals were subjected to a standardized hemorrhagic shock protocol, including pulmonary contusion and liver crush injury. To determine molecular alterations in response to trauma as a result of prefeeding, liver and muscle biopsies in addition to serum and urine samples were obtained at set timepoints throughout the procedure. The samples were prepared and analyzed by NMR spectroscopy.

INSTRUMENT(S): Varian, Bruker

PROVIDER: MTBLS123 | MetaboLights | 2015-01-31

REPOSITORIES: MetaboLights

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			Action	DRS
	Liver_Data.xlsx	Xlsx
	M011112LB.zip	Other
	M011112LS45.zip	Other
	M011112MB.zip	Other
	M011112MS45.zip	Other

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