Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nano-functionalized probiotic treats atherosclerosis via inhibiting intestinal microbiota-TMA-TMAO axis

ABSTRACT:

Many patients are suffering from atherosclerosis without typical risk factors, which can cause severe cardiovascular complications. Trimethylamine N-oxide (TMAO), derived from gut microbes, is a key unconventional contributor to the development of atherosclerosis. Here we present a strategy performed by orally administered nano-functionalized probiotics (PDMF@LGG) to inhibit TMAO through the gut microbiota-trimethylamine (TMA)-TMAO axis. PDMF@LGG, composed of polydopamine-coated Lacticaseibacillus rhamnosus GG and nanoparticles based on a reactive oxygen species (ROS)-responsive polymeric prodrug of fluoromethylcholine (FMC), can promote the retention of probiotics and nanoparticles in the intestine to persistently scavenge elevated ROS and release drugs. This process suppresses TMA production and absorption, lowering plasma TMAO levels. The therapeutic effects on male ApoE-/- mice demonstrate that PDMF@LGG reduces TMAO levels, alleviates atherosclerotic plaque formation, and regulates gut microbial composition and various metabolites. Together, PDMF@LGG represents a potential candidate for atherosclerotic therapy caused by TMAO and broadens the range of treatable atherosclerosis.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12426 | MetaboLights | 2025-10-25

REPOSITORIES: MetaboLights

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	LG1_N.mzML	Mzml
	LG1_P.mzML	Mzml
	LG2_N.mzML	Mzml
	LG2_P.mzML	Mzml
	LG3_N.mzML	Mzml

Items per page:

1 - 5 of 30

Similar Datasets

Genome-wide analysis of gene expression by ileum enterocytes following treatment with probiotic Lactobacillus reuteri

Project description:Analysis of changes in gene expression when probiotics are in the neonatal mouse gut (4 hrs) and after they had been excreted (24 hrs post-gavage)

2011-02-01 | GSE26992 | GEO

Genome-wide analysis of gene expression by ileum enterocytes following treatment with probiotic Lactobacillus reuteri

Project description:Analysis of changes in gene expression when probiotics are in the neonatal mouse gut (4 hrs) and after they had been excreted (24 hrs post-gavage) Total RNA obtained from mouse ileum epithelial cells isolated by laser capture microdissection 4 or 24 hours after a single orogastric gavage with probiotic or vehicle (PBS)

2011-02-01 | E-GEOD-26992 | biostudies-arrayexpress

sc-RNAseq analysis of whole aorta from ApoE-/- atherosclerotic mice after 4 weeks, 10 weeks and 16 weeks of high-fat-diet (HFD) and STAT5-in-1 (S5i) administration

Project description:Atherosclerosis is closely associated with oxidative stress, characterized by the generation of reactive oxygen species (ROS). ROS can serve as a polarization queue depending on localization. We observed that predominantly foam cells elicit high mitochondrial ROS burden in human and mouse atherosclerotic lesions. Our results revealed that STAT5 inhibition predominantly affected Trem2hiGpnbmhi foam cell cluster.

2025-07-25 | GSE283919 | GEO

Chromatin accessibility analysis of human monocyte derived macrophages (hMDM) in response to H2O2 or DMNQ stimulation

Project description:Atherosclerosis is closely associated with oxidative stress, characterized by the generation of reactive oxygen species (ROS). ROS can serve as a polarization queue depending on localization. We observed that predominantly foam cells elicit high mitochondrial ROS burden in human and mouse atherosclerotic lesions. Our results show changes in chromatin accessibility with DMNQ, a mitochondrial-specific ROS inducer, compared to H202 treatment for induction of general ROS.

2025-07-25 | GSE283864 | GEO

probiotic metagenome

Project description:Probiotics products

| PRJNA1086402 | ENA

Gene expression kinetics in Murine Gut wall tissue: Reference (Total RNA Mixture of all samples) vs. Probiotics/Salmonella treated mice

Project description:Transcriptional profiling of mouse gut wall tissue following infection with Salmonella or treatment with Probiotics to see the role of probiotics in preventing salmonella infection through gut mucosal route of mouse.

2018-06-18 | GSE98353 | GEO

Effects of a Probiotic Formulation Based on SF68, Phytosterols, and 6S-5-Methyltetrahydrofolic Acid on Body Composition, Biochemical Parameters, Gut Microbiota, and Gastrointestinal and General Symptoms of Overweight/Obese Subjects: The PrObesity Study

Project description:Obesity and dyslipidemia are increasingly widespread. Considering gut microbiota's (GM) role in energy balance, probiotics represent a promising non-pharmacological approach. This study assessed a probiotic formulation (SF68 + phytosterols + (6S)-5-methyltetrahydrofolic acid) on anthropometric, biochemical, and GM parameters, as well as gastrointestinal and general symptoms, in overweight/obese individuals with low-to-moderate cardiovascular risk. Forty participants (30F, mean age 51.5 ± 11 years) were randomized to receive either the probiotic or placebo daily for 12 weeks. Anthropometric data, questionnaires, and biological samples were collected pre- and post-intervention. While both groups showed significant (p < 0.05) improvements, the probiotic group showed greater reductions in waist circumference (−1.8 cm), fat mass (−1.8 kg; −1.7%), total cholesterol (−11 mg/dL), and LDL-cholesterol (−10.3 mg/dL), along with an increased HDL/LDL ratio (+0.1). Both probiotic and placebo resulted in a significant reduction in HbA1c (−1.7 mmol/mol and −1.5 mmol/mol, respectively). The probiotic formulation also reduced erythrocyte ROS (−29.7%), granulocyte ROS (−28.4%), and plasma nitrite/nitrate (49.5%) and significantly increased erythrocyte glutathione (GSH; +81.9%). Gastrointestinal symptoms improved, and GM analysis showed an increased Evenness index and reduced Lachnospirales abundance after probiotic treatment. These findings suggest the multi-component probiotic formulation may support metabolic and gut health in overweight or obese individuals, although further research is warranted

2025-09-19 | GSE295639 | GEO

probiotic metagenome

Project description:Animal probiotics feed additives metagenome

| PRJNA823500 | ENA

Pharmacokinetic modeling and gene sequencing reveal the interplay between B vitamins and probiotics

Project description:The pharmacological significance of B vitamins, essential for various metabolic processes, and the therapeutic potential of probiotics in gastrointestinal health have been well-documented. However, the interactions between these entities remain poorly understood. In this study, we endeavored to elucidate the potential interplay between B vitamins and probiotics utilizing liquid chromatography-triple quadrupole mass spectrometry, pharmacokinetic modeling, and 16S rRNA gene sequencing. Employing healthy and pseudo-germ-free rat models, we revealed that probiotics significantly improve the absorption of B1, B3, B5, and B12, and that the gut microbiota played a mediating role in this enhanced absorption of B vitamins by probiotics. High-throughput genetic sequencing uncovered a synergistic effect of B vitamins and probiotics in modulating the gut microbiota, particularly increasing the abundance of Verrucomicrobia and Akkermansia. Furthermore, in vitro experiments demonstrated that probiotics used in this study had a relatively minor influence on the production and permeability of B vitamins, while B vitamins did not significantly contribute to the growth, auto-aggregation, and adhesion of probiotics. In summary, a complex network connection exists between B vitamins and probiotics, wherein the gut microbiota emerges as a pivotal factor that cannot be overlooked.

2024-07-31 | GSE253146 | GEO

Maternal Probiotic Supplementation Effects on the Placenta-Brain Axis

Project description:The rate of probiotic usage by pregnant women in the US and Canada ranges from 1.3 to 3.6 %. Probiotic supplements are available without a prescription and have gained currency in treating a variety of ailment ranging from reducing risk of constipation, diarrhea, other gastrointestinal conditions, eczema, pre-term birth, and prevent adverse pregnancy outcomes, including gestational diabetes mellitus (GDM) and depression/anxiety. Three possible mechanisms by which maternal probiotic supplementation might influence the placenta are through 1) directly impacting possible bacteria residing in the placenta (placenta microbiome), 2) altering bacterial metabolites produced by gut microbiota within the mother that induce placental changes, and 3) maternal probiotics might affect the composition of the bacteria within the maternal gut that affects her immune cells and their responses to the heterologous placenta. For the second potential mechanism, bacterial metabolites that might influence placenta include short chain fatty acids (SCFAs), polyamines (PAs), and Vitamins B9 (Folic Acid) and 12 (Cobalamin), among others. This project aims to determine the effects maternal probiotic supplementation in mice might have on the fetal placenta. With the number of women taking over probiotic supplements increasing, further research is needed to determine how these bioactive agents may affect the placenta and health of the offspring.

2025-09-26 | GSE309308 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data