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Multi-omics analysis of Arabidopsis leaves upon infection by necrotrophic pathogen Botrytis cinerea

ABSTRACT:

Botrytis cinerea is a necrotrophic pathogen that induces host cell death, causing substantial economic losses in agriculture. In this study, we show that functional depletion of INOSITOL AUXOTROPHY 80 (INO80), a nuclear factor involved in local chromatin remodeling, reduces the susceptibility of model plant Arabidopsis to this necrotrophic pathogen. Using spatial transcriptomics, a cutting-edge technology enabling simultaneous acquisition of transcriptomes from distinct regions within the same tissue, we provide an unprecedented spatial profiling of the transcriptional response in Arabidopsis leaves following B. cinerea infection. Sequential changes in numerous key genes associated with plant defense pathways were observed across different regions of infected leaves. Additionally, by comparing the phosphoproteome and metabolome of wild-type (WT) and ino80 mutant leaves after B. cinerea infection, we reveal the role of INO80 in regulating multiple hormone pathways and metabolic processes, underscoring the critical importance of chromatin machinery in modulating plant responses to biotic stresses. Collectively, our findings establish a framework for describing, exploring and elucidating spatial information alongside plant multi-molecular responses upon encountering pathogenic challenges.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12467 | MetaboLights | 2026-03-09

REPOSITORIES: MetaboLights

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			Action	DRS
	NEG_QC01.raw	Raw
	NEG_QC02.raw	Raw
	NEG_QC03.raw	Raw
	NEG_WT_0_1.raw	Raw
	NEG_WT_0_2.raw	Raw

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