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Impact of reproductive activity on physiological metabolism and molecular regulation in the Genus scallopus(Mizuhopecten yessoensis)

ABSTRACT:

Genus scallopus(Mizuhopecten yessoensis), a commercially important shellfish species in China, suffers a persistently high post-spawning mortality rate, severely constraining industry development. Thus, this study investigated the physiological and molecular impact of reproductive activity on M. yessoensis. There were significant decreases (P <0.05) in nutritional components (crude protein, crude lipid, and glycogen content) within the gonads before and after spawning, with a particularly pronounced reduction in crude lipids. Histological examination showed that, post spawning, lumens formed within the gonadal follicles of both sexes following gamete release, accompanied by decreased germ cell numbers. Enzyme activity assays indicated significant changes in enzyme activities post spawning. Superoxide dismutase and lactate dehydrogenase exhibited significant differences (P <0.01) in the female mantle. ATPase content displayed a decreasing trend in all tissues of both male and female scallops. Alanine aminotransferase showed significant differences (P <0.01) in the gonads and adductor muscles, and aspartate aminotransferase activity increased significantly (P <0.05) in the adductor muscles, of both male and female scallops. Gonadal metabolomics analysis identified 1,644 differential metabolites between pre- and post-spawning periods, primarily enriched in lipid and amino acid metabolic pathways. Female metabolic changes were concentrated in lipid and amino acid metabolism, whereas males showed alterations in sulfur-containing compound metabolism. In summary, the reproductive process significantly impacts the physiological status and metabolic patterns of M. yessoensis, with distinct variations between sexes. This study provides fundamental data for in-depth analysis of energy allocation during bivalve reproduction and lays a theoretical foundation for exploring the regulatory networks of reproductive metabolism in M. yessoensis at the molecular level.

INSTRUMENT(S): Liquid Chromatography MS - positive - HILIC, Liquid Chromatography MS - negative - HILIC

PROVIDER: MTBLS12652 | MetaboLights | 2025-06-28

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS12652_LC-MS_negative_hilic_metabolite_profiling.txt	Txt
	a_MTBLS12652_LC-MS_positive_hilic_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12652_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS12652_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:In internally fertilizing organisms, mating involves a series of highly coordinated molecular interactions between the sexes that occur within the female reproductive tract. In species with promiscuous mating systems, traits involved in postcopulatory interactions are expected to evolve rapidly, potentially leading to postmating-prezygotic (PMPZ) reproductive isolation between diverging populations. Here, we use a novel study design to investigate the postmating transcriptional response of Drosophila mojavensis female reproductive tracts following copulation with either conspecific or heterospecific (D. arizonae) males at three time points postmating. Relatively few genes (15 total) were transcriptionally regulated in the female lower reproductive tract in response to conspecific mating. Heterospecifically-mated females exhibited significant perturbations in the expression of the majority of these genes, and also downregulated transcription of a number of others, including several involved in mitochondrial function. These striking regulatory differences indicate failed postcopulatory molecular interactions between the sexes consistent with the strong PMPZ isolation observed for this cross. We also report, for the first time, the transfer of male mRNA transcripts to females during copulation. These included transcripts from male accessory-gland proteins (ACPs), a finding with potentially broad implications for understanding postcopulatory molecular interactions between the sexes. Dataset from Postmating transcriptional changes in reproductive tracts of con- and heterospecifically-mated Drosophila mojavensis females Bono, JM, Matzkin,LM, Kelleher, ES, and Markow, MA, Proceedings of the National Academy of Sciences, USA. The treatments were con- and heterospecific mated D. mojavensis females. Three different post-copulation times points were assayed, 15 minutes, 2 hours and 6 hours. For each time point there were two independent replicates. Additionally, two replicates of a virgin D. mojavensis female control were assayed. A total of 14 arrays. All RNA extractions were from the lower reproductive tract of females.

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Impact of reproductive activity on physiological metabolism and molecular regulation in the Genus scallopus(Mizuhopecten yessoensis)

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