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Untargeted metabolomics of offspring rats in MIA model

ABSTRACT:

Maternal immune activation is a significant environmental factor for neurodevelopmental diseases. Although gut bacteria and their metabolites are linked to behavioral deficits in NDDs, the roles of gut fungi and secondary fungal metabolites remain unclear. Here, we induced MIA via a single intraperitoneal injection of 5 mg/kg Poly IC in pregnant rats on gestational day 15 and characterized its effects on mycotoxin profiles in juvenile rat offspring. MIA disturbed the mycotoxin profiles in offspring fece. Specifically, levels‌ of dihydrohydroxy-O-methylsterigmatocystin (Dihydro-HOMST), citreoviridin D, fusaric acid, and wortmannin were elevated in Poly I:C-exposed offspring compared to controls, whereas those of aflatoxin B1 dialcohol and kojic acid levels were reduced. Based on Spearman's correlation analysis, the relative abundance of gut fungi correlated significantly with the levels of mycotoxins produced by these fungi.

INSTRUMENT(S): Liquid Chromatography MS - negative, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12687 | MetaboLights | 2026-01-20

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	a_MTBLS12687_LC-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS12687_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12687_LC-MS_negative__metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS12687_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Mycotoxins are secondary metabolites which are produced by numerous fungi and pose a continuous challenge to the safety and quality of food commodities in South Africa. These toxins have toxicologically relevant effects on humans and animals that eat contaminated foods. In this study, a diagnostic DNA microarray was developed for the identification of the most common food-borne fungi, as well as the genes leading to toxin production. A total of 40 potentially mycotoxigenic fungi isolated from different food commodities, as well as the genes that are involved in the mycotoxin synthetic pathways, were analyzed. For fungal identification, oligonucleotide probes were designed by exploiting the sequence variations of the elongation factor 1-alpha (EF-1 Î±) coding regions and the internal transcribed spacer (ITS) regions of the rRNA gene cassette. For the detection of fungi able to produce mycotoxins, oligonucleotides directed towards genes leading to toxin production from different fungal strains were identified in data available in the public domain. The oligonucleotides selected for fungal identification and the oligonucleotides specific for toxin producing genes were spotted onto microarray slides. The diagnostic microarray developed can be used to identify potentially mycotoxigenic fungi as well as genes leading to toxin production in both laboratory and food samples offering an interesting potential for microbiological laboratories. Keywords: Development of a diagnostic microarray for the identification of potentially mycotoxigenic fungi as well as genes leading to toxin production, 40 food-borne fungi, mycotoxins Development of a diagnostic array for the identification of food-borne fungi and their potential mycotoxin-producing genes. Oligonucleotide probes to be printed onto the array were designed by exploiting the sequence variations of the elongation factor 1-alpha (EF-1 Î±) coding regions and the internal transcribed spacer (ITS) regions of the rRNA gene cassette. For the detection of fungi able to produce mycotoxins, oligonucleotides directed towards genes leading to toxin production from different fungal strains were identified in data available in the public domain. Analysis was performed with 40 fungal cultures were obtained from the Agricultural Research Council culture collection (ARC), Pretoria, South Africa.an in-house spotted oligonucleotide microarray. The identity of each fungus was confirmed by standard laboratory procedures. For DNA isolation, the fungal strains were grown on 1.5% malt extract agar at 25Â°C for 1-2 weeks and total genomic fungal DNA was extracted following the DNA extraction protocol described by Raeder and Broda (1985). The internal transcribed spacer oligonucleotides ITS1, ITS3 and ITS4 were used as a reference for normalization of all spot intensity data.Samples were fluorescently labelled with Cy5 dye by using a Cyâ¢Dye Post-labelling Reactive Dye Pack and wre hybridized to the oligonucleotide microarray overnight. Two biological and one technical replicate (using independent labelling reactions) was performed, each replication consisting of a reverse labelling experiment.

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Untargeted metabolomics of offspring rats in MIA model

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