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Untargeted Metabolomics of Brassica oleracea under Oleamide Supplementation: Resistance-Associated Changes

ABSTRACT:

Oleamide, a fatty acid amide with potential bioactive properties against Xanthomonas campestris pv. campestris (Xcc), was evaluated for its impact on the metabolome of Brassica oleracea. Susceptible 'Early Big' plants at six weeks of age were pretreated with 60 µM oleamide. Twenty-four hours after treatment, plants were inoculated with Xcc race 1 either in the morning (AM) or evening (PM), and leaf tissue was collected 24 hours post-inoculation for untargeted metabolomic analysis. Oleamide supplementation induced substantial shifts in the metabolic profile compared to untreated plants, with pronounced effects in pathways associated with defense responses. Time-of-day-dependent variation in metabolite accumulation was also observed. These results indicate that oleamide modulates the metabolic landscape of B. oleracea, promoting early defense-related metabolic reprogramming during infection.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12902 | MetaboLights | 2026-03-13

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS12902_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS12902_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12902_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS12902_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Diurnal Dynamics Shape Plant Defense Responses: Temporal Variations in Disease Progression and Metabolic Reprogramming in Brassica oleracea

Project description:Plants synchronize their immune responses with the circadian cycle, yet the impact of diurnal timing on disease resistance remains poorly understood in crop species. In this study, we investigated how diurnal variation modulates defense responses in Brassica oleracea against Xanthomonas campestris pv. campestris (Xcc). Using a resistant cabbage variety (Badger Inbred-16) and a susceptible broccoli variety (Early Big), we found that plants inoculated in the evening exhibited significantly reduced disease severity compared to morning infections. Metabolomic and gene expression analyses revealed that the circadian clock orchestrates metabolic reprogramming during infection, influencing key defense-related compounds. Lipid metabolism, particularly the accumulation of fatty acid amides such as oleamide, was strongly associated with evening resistance. Additionally, genes involved in jasmonic acid biosynthesis and energy metabolism were upregulated at night, suggesting a coordinated defense response. Exogenous application of oleamide mimicked the evening resistance phenotype, reducing lesion size and inhibiting bacterial growth in vitro. These findings indicate that diurnal immune regulation is a critical factor in plant-pathogen interactions and that leveraging circadian-driven metabolic shifts could enhance crop disease management strategies.

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