Metabolomics profiling of traditional bioformulations: calotropis ark, dashparni ark and neemboli ark

ABSTRACT:

The growing shift toward sustainable agriculture has intensified interest in traditional bioformulations derived from botanicals and microbial consortia. This study presents an integrative metabolomic profiling of plant-based arks widely used in Indian natural farming practices: Calotropis Ark (CA), Dashparni Ark (DA), and Neemboli Ark (NA). Using untargeted metabolomics (GC-MS and LC-MS), distinct metabolite signatures across formulations were identified. GC-MS based identification revealed that in DA, NA, and CA most abundant class of compounds were organoheterocyclic compounds (22%), benzenoids (21%), and organoheterocyclic compounds (19%), respectively. GC-MS identified 44 core and over 180 unique metabolites across the formulations. Shared compounds in all bioformulations included benzoic acid, 1,3-propanediol, 2-pentenoic acid, D-glucitol, 2-propenoic acid, inositol, and 2-butenoic acid, etc. In LC-MS, the most abundant class of compounds was lipids and lipid-like molecules in NA (33%), DA (32%), and CA (28%), followed by organoheterocyclics and organic acids. LC-MS data showed 619 shared metabolites (e.g., jasmonic acid, benzothiazole, cinnamic acid, octanal, 17-octadecynoic acid, etc.). This will enhance the understanding of composition of such bioformulations in applications to the sustainable agricultural practices.

INSTRUMENT(S): Gas Chromatography MS -, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12925 | MetaboLights | 2026-01-23

REPOSITORIES: MetaboLights