Multi-omics analysis reveals urea promoting Salix matsudana growth and facilitating soil remediation
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ABSTRACT: In this manuscript, we monitored the growth of S. matsudana and measured the biomass of its various organs. Concurrently, we analyzed the rhizosphere metabolome and microbiome. The results demonstrated that urea application promotes both the growth of S. matsudana and the accumulation of heavy metals. Moreover, urea induced significant changes in the rhizosphere microbial community and metabolic profiles. Integrated metabolomic and microbiome analyses further revealed the interactive effects of urea on the metabolic processes within the rhizosphere soil and the resident microbiota. Collectively, this study employs a multi-omics approach to elucidate the mechanism by which urea enhances the growth of S. matsudana and promotes soil remediation.
INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase
PROVIDER: MTBLS12969 | MetaboLights | 2025-09-09
REPOSITORIES: MetaboLights
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Project description:In the soil the stability of urea is affected by the presence of urease, a ubiquitous enzyme released in the rhizosphere by microbial population and by decomposition of organic matter. To reduce the impact on farmer economies and environmental pollution, a common agronomical practice consists of applying urease inhibitors which delays the hydrolysis of urea and, in turn, ammonia is slowly release in the soil. General aim of the present work was the description of changes in maize root transcriptome occurring in response to treatment with the urease inhibitor NBPT.
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Project description:<p> Soil contamination with heavy metals, particularly lead (Pb) and cadmium (Cd), has become a growing environmental concern due to rapid industrialization. Salix matsudana, a woody energy plant with notable tolerance to heavy metals, exhibits considerable potential for use in phytoremediation. Although nitrogen (N) fertilization is known to promote the growth of S. matsudana, its dual role in enhancing plant development and improving soil conditions remains insufficiently understood. In this study, we systematically examined the effects of varying nitrogen fertilizer concentrations on the growth of S. matsudana, heavy metal uptake, and rhizosphere soil characteristics through integrated physiological and multi-omics approaches. Our findings revealed that high nitrogen levels significantly increased plant biomass and the accumulation of Pb and Cd compared to control conditions. Detailed physiological analyses demonstrated enhanced heavy metal absorption in roots under nitrogen fertilization. Microbial community analysis further indicated that nitrogen application altered rhizosphere microbial activity, particularly enriching bacterial taxa associated with metal mobilization. Metabolomic profiling corroborated improvements in soil quality, showing significant changes in organic acid metabolites involved in metal chelation. These results provide new insights into the mechanisms by which nitrogen fertilization synergistically enhances both the growth of S. matsudana and the efficiency of soil remediation, offering valuable guidance for optimizing phytoremediation strategies in heavy metal-contaminated environments.</p>
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