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Strontium chelate with Achyranthes bidentata polysaccharide as a carrier promotes bone regeneration through mediating gut-liver-bone axis: The TD chicken model

ABSTRACT:

This study investigates the effect of Achyranthes bidentata polysaccharide-strontium (ABPS-Sr) chelate on tibial dyschondroplasia (TD) in broilers and its underlying mechanisms. TD is a prevalent leg disorder in commercial broilers characterized by reduced angiogenesis in the tibial growth plate (TGP), with limited effective interventions in modern poultry farming. ABPS-Sr was synthesized as a novel chelate, leveraging Achyranthes bidentata polysaccharide to target lower limb bone repair by delivering osteogenic and angiogenic strontium. In a thiram-induced TD broiler model, ABPS-Sr significantly improved growth performance and tibial bone microstructure, while promoting angiogenesis in the TGP. Mechanistically, ABPS-Sr promoted angiogenesis within the TGP by upregulating the expression of vascular endothelial growth factors and restored osteogenic differentiation by activating the ITGB1/FAK/PI3K/AKT1 signaling pathway. Additionally, ABPS-Sr regulated gut microbiota composition, restored intestinal barrier function, and corrected hepatic lipid metabolism disorders, particularly by reducing plasma glycerophospholipid (e.g., phosphatidylcholine) accumulation that exerts negative effects on bone health. Fecal microbiota transplantation experiments further confirmed that the gut microbiota is essential for mediating the bone-protective effects of ABPS-Sr. Collectively, these findings reveal that ABPS-Sr effectively alleviates TD through direct actions on bone repair and angiogenesis as well as by orchestrating recovery along the gut-liver-bone axis, which provides a promising nutritional intervention strategy for improving poultry bone health.

INSTRUMENT(S): Liquid Chromatography MS - positive - HILIC, Liquid Chromatography MS - negative - HILIC

PROVIDER: MTBLS13045 | MetaboLights | 2025-09-25

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13045_LC-MS_negative_hilic_metabolite_profiling.txt	Txt
	a_MTBLS13045_LC-MS_positive_hilic_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13045_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13045_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:The phases of fracture healing have been well characterized. However, the exact source and genetic profile of the skeletal progenitors that participate in bone repair is somewhat unclear. Sox9 expression in skeletal elements precedes bone and cartilage formation and a Sox9+ cell type is retained in the adult periosteum. We hypothesized that Sox9+ periosteal cells are multipotent skeletal progenitors normally participating in fracture repair. To test this hypothesis we used tamoxifen (TM)-mediated lineage tracing of Sox9+ cells in Sox9CreErt2:Td-Tomato mice. TM injection indelibly labels Sox9+ cells and all their descendants with the fluorescent reporter protein Td-Tomato. Intact mouse femora were harvested 2 weeks after TM injection and analyzed by histology and immunostaining and RNA sequencing, to evaluate the skeletal distribution and gene expression profile of Td-Tomato positive cells in the adult femur. To assess the role of these cells in fracture repair, mice underwent a closed mid-diaphyseal femoral fracture and their hind limbs were harvested at 3, 9 or 56 days post-fracture to assess the contribution of Sox9+ and Td-tomato+ cells in the fracture healing process. In the intact adult mouse femur, Td-Tomato-labelled cells were observed in articular and growth plate cartilage where Sox9 is known to be expressed, but also in the primary spongiosa, periosteum, and endosteum. RNA sequencing and subsequent analysis showed that Td-Tomato positive periosteal cells were enriched in Sox9 transcripts, and transcripts for various osteogenic and chondrocyte specific genes. In a femoral fracture model, we showed that pre-labeled Td-Tomato positive descendent cells were mobilized during the fracture repair process, expanding and migrating towards the fracture site 3 days post-fracture. Here, Td-Tomato positive cells differentiate into chondrocytes and osteoblasts in the soft and hard callus, respectively, 9 days post-fracture. By 2 months post-fracture, descendants of the original Sox9 labelled cell population were prevalent in the cortex and periosteum, and amongst the differentiated osteocyte population embedded within the cortical bone. Thus, a Sox9+ progenitor population resides in the adult periosteum. Fate tracing shows that these cells likely play a substantial role in repair of the fractured femur giving rise to chondrocytes, osteoblasts and mature cortical osteocytes. To our knowledge this is the first report of this Sox9+ cell population in the periosteum of the adult long bone. Taken together with developmental studies on skeletal formation, our data suggest that Sox9+ osteochondroprogenitors play a continuous role in skeletal formation throughout life.

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Strontium chelate with Achyranthes bidentata polysaccharide as a carrier promotes bone regeneration through mediating gut-liver-bone axis: The TD chicken model

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