Dataset Information

Serum metabolomics reveal mechanisms by which fermented brewer’s spent grains promote intestinal development in white-feathered broilers

ABSTRACT:

This study aimed to investigate the effects of wet-fermented brewer's grains (WFBG) on gut development and serum metabolism in white-feathered broilers. A total of 192 one-day-old male broilers (initial body weight: 36.46 ± 0.93 g) were randomly assigned to two treatment groups: the control group (0% WFBG) and the experimental group (20% WFBG inclusion), with six replicates of 16 birds per replicate. The results of intestinal morphological parameters quantified using ImageJ software after hematoxylin-eosin (HE) staining showed that compared with the control group, broilers fed a diet supplemented with 20% WFBG had significantly improved duodenal development. Specifically, the duodenal villus height (VH) increased by 10.2% (P < 0.05), and the villus height-to-crypt depth ratio (VH/CD) increased by 27.2% (P < 0.05)—both indicators reflecting the enhanced duodenal development. Through untargeted metabolomics analysis for screening differentially-expressed metabolites (DEMs) of serum samples, 211 DEMs were identified, including 98 upregulated DEMs and 113 downregulated DEMs in the WFBG group. KEGG pathway enrichment analysis revealed that these DEMs were significantly associated with key metabolic processes, including linoleic metabolic pathways, linoleic acid metabolism, phenylalanine metabolism, and other relevant pathways. Specifically, key DEMs involved in amino acid metabolism showed significantly increased phenylalanine levels and decreased 4-HPA and 3-HPA levels (P < 0.05). In conclusion, the inclusion of 20% WFBG in the diet of white-feathered broilers significantly promoted intestinal development. These favorable outcomes tie to modified serum metabolic profiles and adjustments to lipid/amino acid metabolism in broilers, underscoring WFBG’s significance for boosting broiler intestinal development while also adding to the theoretical framework for poultry by-product-based feeds.

INSTRUMENT(S): Liquid Chromatography MS - - -, Liquid Chromatography MS - - - Thermo Orbitrap Exploris 120

PROVIDER: MTBLS13078 | MetaboLights | 2025-09-30

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13078_LC-MS_negative_hilic_metabolite_profiling.txt	Txt
	a_MTBLS13078_LC-MS_positive_hilic_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13078_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13078_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Submerged cultivation using low-value agro-industrial side streams allows large-scale and efficient production of fungal mycelia, which has a high nutritional value. As the dietary properties of fungal mycelia in poultry are largely unknown, the present study aimed to investigate the effect of feeding a Pleurotus sapidus (PSA) mycelium as a feed supplement on growth performance, composition of the cecal microbiota and several physiological traits including gut integrity, nutrient digestibility, liver lipids, liver transcriptome and plasma metabolome in broilers. 72 male, 1-day-old Cobb 500 broilers were randomly assigned to three different groups and fed three different adequate diets containing either 0% (PSA-0), 2.5% (PSA-2.5) and 5% (PSA-5.0) P. sapidus mycelium in a three-phase feeding system for 35 days. Each group consisted of 6 cages (replicates) with 4 broilers/cage. Body weight gain, feed intake and feed:gain ratio and apparent ileal digestibility of crude protein, ether extract and amino acids were not different between groups. Metagenomic analysis of the cecal microbiota revealed no differences between groups, except that one α-diversity metric (Shannon index) and the abundance of two low-abundance bacterial taxa (Clostridia UCG 014, Eubacteriales) differed between groups (P < 0.05). Concentrations of total and individual short-chain fatty acids in the cecal digesta and concentrations of plasma lipopolysaccharide and mRNA levels of proinflammatory genes, tight-junction proteins, and mucins in the cecum mucosa did not differ between groups. None of the plasma metabolites analyzed using targeted-metabolomics differed across the groups. Hepatic transcript profiling revealed a total of 144 transcripts to be differentially expressed between group PSA-5.0 and group PSA-0 but none of these genes was regulated greater 2-fold. Considering either the lack of effects or the very weak effects of feeding the P. sapidus mycelium in the broilers it can be concluded that inclusion of a sustainably produced fungal mycelium in broiler diets at the expense of other feed components has no negative consequences on broilers´ performance and metabolism.

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Serum metabolomics reveal mechanisms by which fermented brewer’s spent grains promote intestinal development in white-feathered broilers

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