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Integrating Blood Microsampling Devices with CE-MS for Untargeted Analysis of Polar Metabolomics

ABSTRACT:

Capillary electrophoresis–mass spectrometry (CE-MS) offers considerable advantages for polar compound metabolomics analysis, including simple workflows, fast method development, and suitability for small sample volumes. Blood microsampling (BμS) techniques (such as dried blood spots, quantitative DBS, and volumetric absorptive microsampling) are minimally invasive and have gained increased attention over the past years. Despite this growing interest, few studies have compared the metabolic profiles derived from these sampling methods and, to our best knowledge, none have employed CE-MS.

The aim of this study is to evaluate the potential of CE-MS as an analytical technique to characterize and compare the polar metabolomic profiles obtained from the three tested BμS devices to those of whole blood and plasma, which is the most used blood specimen. We compared extraction protocols based on their recovery, precision, and matrix effects for a panel of stable isotopically-labeled amino acids and acylcarnitines. Most labeled compounds showed satisfactory recovery and precision, although some matrix effects were observed in BμS device extracts.

Finally, the chosen protocol was used to assess the performance of the devices through the untargeted metabolomic analysis of samples from 10 healthy individuals. All the three BμS devices yielded very similar profiles that closely resembled that of whole blood, though remaining distinguishable from it, and from plasma. Altogether 150 compounds were annotated using a publicly available database, and differences between each BμS device, plasma and whole blood were assessed. Overall, these devices have shown to be convenient alternatives to conventional sampling in untargeted CE-MS metabolomic profiling of blood.

INSTRUMENT(S): Capillary electrophoresis MS - positive, Capillary electrophoresis MS - negative

PROVIDER: MTBLS13090 | MetaboLights | 2026-01-13

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13090_CE-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS13090_CE-MS_positive__metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13090_CE-MS_negative__metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13090_CE-MS_positive__metabolite_profiling_v2_maf.tsv	Tabular

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Integrating Blood Microsampling Devices with CE-MS for Untargeted Analysis of Polar Metabolomics

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