Intra-tumoral microbial community profiling and associated metabolites alterations of TNBC

ABSTRACT:

Triple-negative breast cancer (TNBC) presents significant challenges to female

health owing to the lack of therapeutic targets and its poor prognosis. In recent

years, in the field of molecular pathology, there has been a growing focus on the

role of intra-tumoral microbial communities and metabolic alterations in tumor

cells. However, the precise mechanism through which microbiota and their

metabolites influence TNBC remains unclear and warrants further investigation.

In this study, we analyzed the microbial community composition in various

subtypes of breast cancer through 16S rRNA MiSeq sequencing of formalin-fixed,

paraffin-embedded (FFPE) tissue samples. Notably, Turicibacter, a microbe

associated with cancer response, exhibited a significantly higher abundance in

TNBC. Similarly, mass spectrometry-based metabolomic analysis revealed

substantial differences in specific metabolites, such as nutriacholic,

pregnanetriol, and cortol. Furthermore, we observed significant correlations

between the intra-tumoral

INSTRUMENT(S): Liquid Chromatography MS - positive - HILIC, Liquid Chromatography MS - negative - HILIC

PROVIDER: MTBLS13119 | MetaboLights | 2025-10-12

REPOSITORIES: MetaboLights