Enhanced bioproduction of phenolic compounds from Populus nigra L. using temporary immersion bioreactors and metabolomics

ABSTRACT:

Black poplar is a woody species rich in bioactive phenolic compounds with promising pharmaceutical and cosmetic applications. In wild populations, genetic diversity and environmental variability affect phenolic content and bioactivities. Plant tissue culture, conducted under controlled conditions, offers a suitable alternative for industrial bioproduction. The aim of this study was the development of in vitro shoot cultures of black poplar in MagentaTM and RITA® systems for efficient and constant phenolic productivity. Following the initiation of in vitro lines, significant growth enhancement was achieved through line selection. UPLC-QTOF-MS-based untargeted metabolomic analyses were carried out, and allowed the identification of flavan-3-ols, proanthocyanidins, flavonols and salicinoids. As a result, 32 compounds were described for the first time in P. nigra, including 15 metabolites previously identified in other Populus species and 17 additional compounds not yet identified in the Populus genus. The productivity of the major phenolic compounds was substantially higher in the RITA® system, showing a 2.6-fold increase compared to MagentaTM. Targeted metabolomics followed by Principal Component Analysis were performed to study the metabolic changes during 8 weeks of culture in RITA® system. Optimal shoot growth and phenolic content were achieved in RITA® system after 3 weeks of culture. Finally, the effects of both SA and MeJa treatments were characterized by Orthogonal Partial Least Squares Discriminant Analysis and identified flavan-3-ols and proanthocyanidins as biomarkers of both elicitation. In conclusion, in vitro shoot culture in RITA® system and metabolomics studies allowed to design a specific process for efficient bioproduction of black poplar bioactive phenolics.

INSTRUMENT(S): Diode array detection MS - negative - reverse phase

PROVIDER: MTBLS13131 | MetaboLights | 2025-11-06

REPOSITORIES: MetaboLights