Staphylococcus aureus coagulases increase survival of Pseudomonas aeruginosa during coinfection

ABSTRACT:

Polymicrobial infections involving Staphylococcus aureus are often more severe than single-species infections. S. aureus typically initiates the infection, creating conditions that allow secondary pathogens like Pseudomonas aeruginosa to establish. In this study, we investigate how coagulase production and the resulting clots contribute to the persistence of polymicrobial infections and influence interactions between these pathogens. Our results reveal a systemic metabolic shift in both bacteria when cocultured. We also find that P. aeruginosa can benefit from the coagulases produced by S. aureus. P. aeruginosa displays enhanced survival in an in vitro clinical model and ex vivo samples of human blood where coagulases are available. Using a murine model of infection, we demonstrate that coagulases also contribute towards the hematogenous spread and persistence of P. aeruginosa in organ tissues. Taken together, we elucidate why S. aureus and P. aeruginosa coinfections are recalcitrant and result in worse clinical outcomes. 

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - HILIC, Liquid Chromatography MS - positive - reverse phase, Liquid Chromatography MS - negative - HILIC

PROVIDER: MTBLS13310 | MetaboLights | 2026-10-16

REPOSITORIES: MetaboLights