Rv3400 is a phosphoglucomutase required for trehalose metabolism in Mycobacterium tuberculosis

ABSTRACT:

Mycobacterium tuberculosis is a global killer causing over a million deaths from tuberculosis (TB) every year. It is therefore a major burden on human health. To reduce the deadly impact of TB, we need a better understanding of the strategies used by M. tuberculosis to adapt its metabolism to survive and persist in the human host. This will help us design better strategies for TB treatment and control. Previous enzymological studies have reported that the Mtb rv3400 gene encodes a β-phosphoglucomutase; however, its role in M. tuberculosis metabolism has not been investigated. In the present study, we show that deletion of rv3400 leads to a 30-fold increase in β-D-glucose 1-phosphate, confirming its primary function as a β-phosphoglucomutase. Additionally, deletion of rv3400 leads to a growth defect when trehalose is the sole carbon source, indicating that this enzyme is required for optimal use of trehalose, an essential disaccharide in mycobacteria. 

INSTRUMENT(S): Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS13438 | MetaboLights | 2026-08-07

REPOSITORIES: MetaboLights