Dataset Information

Traditional Chinese medicine Dachaihu decoction alleviates abnormal behavior through regulating gut microbiota and metabolites in children with ASD

ABSTRACT:

Traditional Chinese Medicine Dachaihu decoction (DCHD) has been reported to hold great promise to alleviate autism spectrum disorders (ASD), a generalized neurodevelopmental disease associated with gut microbiome dysbiosis. Health-promoting effects of DCHD on ASD children could be attributed to gut microbiota reconstruction. However, its underlying mechanism of action remains elusive, resulting in rare therapies have proposed for TCM-targeted modulation of gut microbiota for the treatment of ASD. This study aimed to investigate the mechanisms by which DCHD alleviates ASD severity and social disorder, with particular emphasis on gut microbiota composition and metabolism.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS13501 | MetaboLights | 2025-12-12

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13501_LC-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS13501_LC-MS_negative_reverse-phase_metabolite_profiling-1.txt	Txt
	a_MTBLS13501_LC-MS_positive__metabolite_profiling.txt	Txt
	a_MTBLS13501_LC-MS_positive_reverse-phase_metabolite_profiling-1.txt	Txt
	i_Investigation.txt	Txt

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Project description:The novel Traditional Chinese Medicine Ramulus Mori (Sangzhi) alkaloid tablets (SZ-A) are approved by The China National Medical Products Administration for the treatment of type 2 diabetes mellitus (T2DM). However, the extensive pharmacological characteristics and the underlying mechanism are unknown. This study investigated the mechanisms by which SZ-A ameliorates glucose metabolism in KKAy mice, an animal model of T2DM. Diabetic KKAy mice were treated intragastrically with SZ-A once daily for 8 weeks, after which glucose levels, lipid metabolism, gut microbiome, systemic inflammatory factors, luminal concentrations of short-chain fatty acids (fecal samples), and ileal proteomic changes were evaluated. The ileum tissues were collected, and the effects of SZ-A on pathological inflammatory damage were evaluated by hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry. The mRNA and protein expression levels of various inflammatory markers, including monocyte chemoattractant protein-1 and phosphorylated nuclear factor kappa B p65, were detected in the ileum tissues. SZ-A improved glucose metabolism with enhanced insulin response and elevated glucagon-like peptide 1 (GLP-1) during the glucose tolerance test in diabetic KKAy mice. Gut microbiota analysis demonstrated that SZ-A administration elevated the abundance of Bacteroidaceae and Verrucomicrobia, reduced the levels of Rikenellaceae and Desulfovibrionaceae; and increased the concentrations of fecal acetic and propionic acids compared to the diabetic model group. Additionally, SZ-A markedly improved ileal inflammatory injury and pro-inflammatory macrophage infiltration and improved intestinal mucosal barrier function in diabetic KKAy mice. SZ-A also attenuated the levels of circulating endotoxin, pro-inflammatory cytokines, and chemokines in the mice sera. Collectively, SZ-A ameliorated the overall metabolic profile including glucose and lipid metabolism in KKAy mice, which may be associated with an improvement in GLP-1 and insulin secretion, at least in part by modulating the gut microbiome and relieving the degree of ileal and systemic inflammation.

Dataset Information

Traditional Chinese medicine Dachaihu decoction alleviates abnormal behavior through regulating gut microbiota and metabolites in children with ASD

Dataset's files

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