Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Discover the Maze-like Pathway for Glabridin Biosynthesis

ABSTRACT:

Glabridin is a bioactive isoflavan predominantly produced in Glycyrrhiza species and is of significant pharmacological and industrial interest. However, its biosynthetic pathway remains incompletely resolved due to the involvement of multiple branching reactions and poorly characterized enzymatic steps. In this study, we employed untargeted and targeted LC–MS-based metabolomics to systematically characterize intermediates and pathway dynamics associated with glabridin biosynthesis. Metabolite profiling was performed across plant tissues from multiple Glycyrrhiza species to capture pathway-associated metabolite variation. The resulting dataset provides comprehensive mass spectrometric evidence supporting a maze-like biosynthetic route toward glabridin formation and offers a valuable resource for pathway elucidation and specialized metabolism research.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS13519 | MetaboLights | 2026-01-16

REPOSITORIES: MetaboLights

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	a_MTBLS13519_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13519_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS13519.txt	Txt

Items per page:

1 - 4 of 4

Similar Datasets

Calotropis Procera

Project description:Transcript analysis of Calotropis procera seedling for elucidation of biosynthetic pathway

| PRJNA257023 | ENA

Expression level of an industrial yeast strain producing isobutanol and wild-type

Project description:Microarray analysis performed with an evolved strain derived from the industrial strain EthanolRed for accession of the differential expression level of the strain with the biosynthetic pathway for production of isobutanol versus the wild-type strain.

2017-04-10 | E-MTAB-5682 | biostudies-arrayexpress

Project description:Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for a-Diazoester Production

| PRJNA968020 | ENA

Discovery of cephalotaxinone enzymes reveals a whole plant model for homoharringtonine biosynthesis

Project description:Plants produce diverse molecules that inhibit protein translation. A lead example is homoharringtonine (HHT), both a key tool for ribosomal profiling and an FDA-approved treatment for chronic myeloid leukemia. HHT is commercially produced through semi-synthesis by esterifying the alkaloid core cephalotaxine (CET) extracted from endangered Cephalotaxus species. Despite its medicinal significance, a biosynthetic pathway to CET and HHT has not been described. Here, we use paired untargeted metabolomics (stable-isotope labeled precursor feeding) and transcriptomics to elucidate a near-complete biosynthesis to CET without prior knowledge of intermediates and biosynthetic genes. We show that while CET alkaloid core is actively biosynthesized only in growing root tips, both CET and HHT accumulate throughout the plant. We discovered and characterized seven CET pathway intermediates and six novel biosynthetic enzymes that can be used to produce cephalotaxinone, the likely direct precursor of CET. Included are non-canonical cytochrome P450s, an atypical short-chain dehydrogenase, and a 2-oxoglutarate-dependent dioxygenase that together result in carbon excision and pentacyclic backbone formation of HHT alkaloids. This study establishes a metabolic route to the core scaffold of HHT and suggests a whole plant coordination model for biosynthesis of eukaryotic ribosomal toxins in Cephalotaxus, where cephalotaxinone is the last pathway intermediate produced in root tips and distributed throughout the plant for subsequent elaboration to HHT.

2026-02-25 | GSE319274 | GEO

Genome analysis and elucidation of the biosynthetic pathway for the cRAS inhibitor rasfonin in Cephalotrichum gorgonifer

Project description:Genome analysis and elucidation of the biosynthetic pathway for the cRAS inhibitor rasfonin in Cephalotrichum gorgonifer

| PRJNA898371 | ENA

Project description:Genomic and biosynthetic diversification of Streptomyces bacteria from multiple bat species and caves

| PRJNA1010360 | ENA

Corynebacterium glutamicum ATCC 13032

Project description:Industrial producer of multiple amino acids

| PRJNA13760 | ENA

Corynebacterium glutamicum ATCC 13032

Project description:Industrial producer of multiple amino acids

| PRJNA307 | ENA

GNPS-neoflavonoid-fanshengxian

Project description:Exploring the mass spectrometric cleavage pathway of neoflavonoid in Dalbergia

2024-06-30 | MSV000095196 | GNPS

Systems-level Analysis of Oxygen Exposure in Zymomonas mobilis: Implications for Isoprenoid Production

Project description:Zymomonas mobilis is an aerotolerant anaerobe and prolific ethanologen with attractive characteristics for industrial bioproduct generation. However, there is currently insufficient knowledge of the impact that environmental factors have on flux through industrially relevant biosynthetic pathways. Here, we examine the effect of oxygen exposure on metabolism and gene expression in Z. mobilis by combining targeted metabolomics, mRNA sequencing, and shotgun proteomics. We found that exposure to oxygen profoundly influenced metabolism, inducing both transient metabolic bottlenecks and long-term metabolic remodeling. In particular, oxygen induced a severe but temporary metabolic bottleneck in the methyl erythritol 4-phosphate pathway for isoprenoid biosynthesis caused by oxidative damage to the iron-sulfur co-factors of the final two enzymes of the pathway. This bottleneck was resolved with minimal changes in expression of isoprenoid biosynthetic enzymes. Instead, it was associated with pronounced upregulation of enzymes related to iron-sulfur cluster maintenance and biogenesis (i.e., flavodoxin reductase and the suf operon). We also detected major changes in glucose utilization in the presence of oxygen. Specifically, we observed increased gluconate production following exposure to oxygen, accounting for 18% of glucose uptake. Our results suggest that under aerobic conditions, electrons derived from the oxidation of glucose to gluconate are diverted to the electron transport chain where they can minimize oxidative damage by reducing reactive oxygen species such as H2O2. This model is supported by the simultaneous upregulation of three membrane-bound dehydrogenases, cytochrome c peroxidase, and a cytochrome bd oxidase following exposure to oxygen.

2019-01-21 | GSE125123 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data