Dataset Information

Integrated multi-omics reveals Enterococcus faecalis JM263 alleviates high-concentrate diet-induced hepatic injury via the gut-liver axis in goats

ABSTRACT:

This dataset contains raw liquid chromatography-tandem mass spectrometry (LC-MS/MS) data derived from the liver tissues of goats (Capra hircus). The purpose of this study is to explore the metabolic regulatory mechanism of the probiotic Enterococcus faecalis JM263 in goats fed a high-concentrate diet (HCD).

Experimental Design

Feeding experiments were conducted on yaks, divided into two groups: 1) Control group, fed a high-concentrate diet; 2) Treatment group, fed the same HCD supplemented with Enterococcus faecalis JM263. After intervention, liver tissues were collected, and metabolomic analysis was performed following quenching and extraction.

Objectives

Untargeted metabolomics was used to identify differential metabolites and enriched pathways related to the hepatoprotective effect of the probiotic. This dataset supports the findings regarding the gut-liver axis interaction in ruminants under dietary stress.

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS13688 | MetaboLights | 2026-01-15

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	a_MTBLS13688_LC-MS_negative_hilic_metabolite_profiling.txt	Txt
	a_MTBLS13688_LC-MS_positive_hilic_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13688_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13688_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Maize and soybean meal are widely used in monogastric animal diets. However, their production raises sustainability concerns. Microalgae, like Chlorella vulgaris, are a potential alternative feedstuff due to their interesting nutritional profile, particularly a high crude protein content. As part of a study investigating the individual and combined effects of dietary C. vulgaris and probiotic Enterococcus lactis SF68 on weaning piglet growth performance, dietary utilization, digestibility, organ morphometrics and physiology by metabolomics, lipidomics and proteomics, the submitted dataset includes the proteomics approach. At weaning (28 days old), Piétrain x (Landrace x Large White) piglets were obtained from a commercial farm. Animals were housed in groups of three in 2m2 pens and, for five weeks, fed one of three diets ad libitum: a control diet based on cereals and soybean meal (Control); a diet with 5% C. vulgaris (CV5); and a diet with 5% C. vulgaris and E. lactis SF68 probiotic (CV5P). Liver, ileum, and muscle tissues were sampled and proteomic analysis performed by LC-MS/MS. Proteomics analysis identified 1966 proteins in the liver, 1592 in the intestine and 1020 in the muscle. In general, multivariate analyses were able to identify broad proteome differences in the liver, but not in the intestine nor muscle. Nonetheless, all organs exhibited notable changes in levels of individual proteins by univariate analyses. Analyses of differential abundance revealed that the probiotic was a major driver of the observed effects whereas the combination of C. vulgaris and probiotic resulted in fewer differentially abundant proteins than expected from an additive effect of individual factors. This suggests the existence of complex interactions between the two dietary components. Overall, results favor the combination of C. vulgaris with the probiotic. In the liver, the combined diet effect resulted in immune attenuation as well as maintained protein catabolism, attenuated mobilization of lipid reserves, and modulation of cellular activity. These effects could enhance the systemic availability of amino acids and lipids to support muscle development during the post-weaning growth stage. In the intestine, evidence of lower ketone oxidation suggests an improved nutritional state, along with evidence of immune priming against potential bacterial infections in piglets fed the CV5P diet. In the muscle, the combined diet effect drives changes in muscle fiber types by affecting connective tissue, mitochondrial respiration and regulation of lipid composition. These findings suggest a positive outcome in expected meat quality, potentially improving tenderness and nutritional value. The output of this dataset discloses the importance of considering the intricate interplay between dietary components and their combined effects on multi-organ systems.

Dataset Information

Integrated multi-omics reveals Enterococcus faecalis JM263 alleviates high-concentrate diet-induced hepatic injury via the gut-liver axis in goats

Dataset's files

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