Dataset Information

Initial exploration of the health effects on Qinghai-Tibetan Plateau yaks following short-term exposure to polystyrene microplastics: analysis of rumen microbiota, host metabolism, antioxidant function and inflammatory responses.

ABSTRACT:

Abstract: Microplastics (MPs) are omnipresent in the environment, including the Qinghai-Tibet Plateau. Previous research on MPs has primarily focused on aquatic species and rodents, with little known about their impact on yaks. This study introduced yak as an animal model, employing a combined analysis of metagenomics and metabolomics to investigate the effects of short-term polystyrene-MPs (PS-MPs) exposure on yak rumen microbiota composition, host metabolism, antioxidant function, and inflammatory responses. Results indicated that PS-MPs exposure altered yak rumen microbiota composition, with significant increases in β-diversity. Four KEGG pathways including peptidoglycan synthesis, vitamin B6 metabolism, riboflavin metabolism, and terpenoid backbone biosynthesis were enriched, and the composition of extracellular polysaccharides was changed. Serum metabolomics revealed elevated L-glutamine and indole-3-propionic acid, coupled with reduced 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and indole-3-lactic acid post-exposure. Urinary metabolomics additionally revealed decreased D-erythrose 4-phosphate, dimethyl allyl pyrophosphate, and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate, collectively indicating inhibited terpenoid backbone biosynthesis in yaks. Additionally, PS-MPs triggered inflammatory responses, evidenced by elevated levels of pro-inflammatory cytokines (interferon-γ, interleukin-1β, interleukin-6, interleukin-17, interleukin-22, tumor necrosis factor-α, transforming growth factor-α), whereas antioxidant function indexes (total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde) showed no significant changes. Multi-omics integration identified Prevotella ruminicola as a pivotal rumen microbe involved in resisting PS-MP invasion. In summary, due to the protective effect of rumen microorganisms, yaks are relatively less affected by PS-MPs invasion in the short term. However, the impacts of long-term MPs exposure on yak organisms and MPs deposition status, as well as the underlying molecular mechanisms warrant further investigation.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS13707 | MetaboLights | 2026-01-17

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	a_MTBLS13707_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13707_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS13707.txt	Txt

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Project description:Microplastics (MPs) are omnipresent in the environment, including the Qinghai-Tibet Plateau. Previous research on MPs has primarily focused on aquatic species and rodents, with little known about their impact on yaks. This study introduced yak as an animal model, employing a combined analysis of metagenomics and metabolomics to investigate the effects of short-term polystyrene-MPs (PS-MPs) exposure on yak rumen microbiota composition, host metabolism, antioxidant function, and inflammatory responses. Results indicated that PS-MPs exposure altered yak rumen microbiota composition, with significant increases in β-diversity. Four KEGG pathways including peptidoglycan synthesis, vitamin B6 metabolism, riboflavin metabolism, and terpenoid backbone biosynthesis were enriched, and the composition of extracellular polysaccharides was changed. Serum metabolomics revealed elevated L-glutamine and indole-3-propionic acid, coupled with reduced 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and indole-3-lactic acid post-exposure. Urinary metabolomics additionally revealed decreased D-erythrose 4-phosphate, dimethyl allyl pyrophosphate, and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate, collectively indicating inhibited terpenoid backbone biosynthesis in yaks. Additionally, PS-MPs triggered inflammatory responses, evidenced by elevated levels of pro-inflammatory cytokines (interferon-γ, interleukin-1β, interleukin-6, interleukin-17, interleukin-22, tumor necrosis factor-α, transforming growth factor-α), whereas antioxidant function indexes (total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde) showed no significant changes. Multi-omics integration identified Prevotella ruminicola as a pivotal rumen microbe involved in resisting PS-MP invasion. In summary, due to the protective effect of rumen microorganisms, yaks are relatively less affected by PS-MPs invasion in the short term. However, the impacts of long-term MPs exposure on yak organisms and MPs deposition status, as well as the underlying molecular mechanisms warrant further investigation.

Project description:BACKGROUND: Grazing yearly on pasture is a traditional practice for yaks, which cannot meet the requirements of yak production since the insufficient forage supplied in the cold season results in a long production cycle. An intensive feeding system increasing production efficiency has been selected for beef and dairy cattle. However, its impacts on yaks are less studied, and it is unclear how the rumen microbiome, rumen metabolites and the host metabolome respond to an intensive feeding system and contribute to yak growth. Here, multi-omics, including rumen metagenomics, rumen and plasma metabolomics, were performed to classify the effects and regulatory mechanisms of intensive feeding system on yaks. RESULTS: In our results, increased growth performance and rumen volatile fatty acid (VFA) concentration were observed in yaks under the intensive feeding system compared to yaks grazing on pastures. Metagenomics of the rumen microbiome revealed that species of Clostridium and Methanobrevibacter as well as Piromyces sp. E2 and Anaeromyces robustus were increased in the rumen of intensively fed yaks, interacting and contributing to amino acid and carbohydrate metabolism. The rumen of yaks grazing on pasture had more cellulolytic microbes, such as Bacteroides and Fibrobacter species. Moreover, yaks under the intensive feeding system had lower methanogens and increased methane degradation functions, suggesting that the methane emission of these yaks may be decreased. These abundant microbiomes were correlated with the pathways of 'Alanine aspartate and glutamate metabolism' and 'Pyruvate metabolism'. Similar with rumen VFA results, metabolomics found that intensively fed yaks had greater concentrations of metabolites related to carbohydrates. The methyl metabolites associated with methane production were greater in the rumen of yak grazing on pasture. Additionally, these changed rumen microbiomes and their metabolites resulted in changes in plasma metabolome, finally influencing yaks’ growth. CONCLUSIONS: This study compressively classifies the mechanism that an intensive feeding system benefits yak growth and reveals the importance of the rumen microbiome for host metabolism and performance. These findings evidence that an intensive feeding system could be used for the yak industry.

Project description:Background: Grazing yearly on pasture is a traditional practice for yaks, which cannot meet the requirements of yak products since the insufficient forage supplied in the cold season results in a long production cycle. An intensive feeding system increasing production efficiency has been selected for beef and dairy cattle. However, its impacts on yaks are less studied, and it is unclear how the rumen microbiome, rumen metabolites and the host metabolome respond to an intensive feeding system and contribute to yak growth. Here, multi-omics, including rumen metagenomics, rumen and plasma metabolomics, were performed to classify the effects and regulatory mechanisms of intensive feeding system.Results: In our results, increased growth performance and rumen volatile fatty acid (VFA) concentration were observed in yaks under the intensive feeding system compared to yaks grazing on pastures. Metagenomics of the rumen microbiome revealed that species of Clostridium and Methanobrevibacter as well as Piromyces sp. E2 and Anaeromyces robustus were increased in the rumen of intensively fed yaks, interacting and contributing to amino acid and carbohydrate metabolism. The rumen of yaks grazing on pasture had more cellulolytic microbes, such as Bacteroides and Fibrobacter species. Moreover, yaks under the intensive feeding system had lower methanogen and increased methane degradation functions, suggesting that the methane emission of these yaks may be decreased. These abundant microbiomes were correlated with the pathways of “Alanine aspartate and glutamate metabolism” and “Pyruvate metabolism”. Similar with rumen VFA results, metabolomics found that intensively fed yaks had greater concentrations of metabolites related to carbohydrates. The methyl metabolites associated with methane production were greater in the rumen of yak grazing on pasture. Additionally, these changed rumen microbiomes and their metabolites resulted in changes in plasma metabolome, finally affecting yaks’ growth.Conclusions: This study compressively classifies the mechanism that an intensive feeding system benefits yak production and reveals the importance of the rumen microbiome for host metabolism and performance. These findings evidence that an intensive feeding system could be used for the yak industry.

Dataset Information

Initial exploration of the health effects on Qinghai-Tibetan Plateau yaks following short-term exposure to polystyrene microplastics: analysis of rumen microbiota, host metabolism, antioxidant function and inflammatory responses.

Dataset's files

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