Real-world neonicotinoid mixture exposure reveals synergistic cytotoxicity and divergent pathway vulnerabilities in human cell lines

ABSTRACT:

In this study, five human cell lines (A2780, Chang liver, HEK293T, HeLa, and HepG2) were used as models and exposed to mixtures of neonicotinoids (NEOs) and their metabolites. The mixture concentrations were prepared based on human biomonitoring data to reflect the actual detected levels of NEOs and their metabolites in human urine. Each cell line was treated with the corresponding human biomonitoring-matched mixture at specified concentrations, alongside a 0.1% DMSO solvent control group. After 48 hours of exposure, intracellular metabolites were extracted and subjected to untargeted metabolomic analysis using an Orbitrap Exploris 120 mass spectrometer in both positive and negative ion modes. During the acquisition process, pooled quality control samples (prepared by mixing equal volumes of all samples) were interspersed to monitor system stability.

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS13896 | MetaboLights | 2026-06-22

REPOSITORIES: MetaboLights