Metabolomic profiling of colon tissue in loperamide-induced constipation mice treated with Rothia mucilaginosa

ABSTRACT:

Untargeted metabolomics was performed on colon tissue from 26 C57BL/6 mice to investigate metabolic mechanisms underlying Rothia mucilaginosa-mediated constipation alleviation. Mice were divided into normal control (NC, n=8), loperamide-induced constipation model control (MC, n=8), and R. mucilaginosa gavage-treated (RG, n=10) groups. The experimental protocol consisted of adaptation (days 0-7), loperamide modeling (days 8-21), and R. mucilaginosa intervention (days 22-40, BNCC363031, 2x10^8 CFU/day). Colon tissue metabolomics was performed using UHPLC-MS/MS (Vanquish UHPLC coupled with Orbitrap Exploris 120, Thermo Fisher) in both positive and negative ionization modes. This dataset supports the identification of differential metabolites and metabolic pathways associated with R. mucilaginosa intervention in constipation, with particular focus on tryptophan metabolism, bile acid metabolism, and neuroactive signaling.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS13907 | MetaboLights | 2026-04-27

REPOSITORIES: MetaboLights