High-confidence untargeted lipidomics of mammalian serum using LC-TIMS-PASEF

ABSTRACT:

This study aimed to establish and validate a robust untargeted lipidomics workflow for the analysis of human serum using reversed-phase liquid chromatography coupled to trapped ion mobility spectrometry time-of-flight mass spectrometry (RP-LC-TIMS-TOF MS). Commercial pooled human serum was used as a standardized biological matrix to evaluate lipid extraction, chromatographic separation, ion mobility separation, and data-processing performance.

INSTRUMENT(S): Liquid Chromatography MS - positive, Liquid Chromatography MS - negative

PROVIDER: MTBLS14002 | MetaboLights | 2026-05-26

REPOSITORIES: MetaboLights