Dataset Information

Trichoderma-based fertilizer enhances quality of Elymus breviaristatus silage via microbial and metabolic modulation

ABSTRACT:

Elymus breviaristatus is an important alpine forage, there is limited information regarding its potential use as silage and how fertilizer treatments affect the ensiling process in this forage crop. Here, we investigated how organic fertilizer (M), nitrogen-phosphorus-potassium fertilizer (NPK), and Trichoderma harzianum fertilizer (B) affect E. breviaristatus silage quality (30 days and 60 days ensiling) via microbiome and metabolome analyses. Before ensiling, plant height and chlorophyll content increased by 29.93% and 39.72%, respectively, in the B group. After 60 days of ensiling, the M group had reduced crude protein and elevated butyric acid, the NPK group had higher ammonia nitrogen and butyric acid, and the B group had increased crude protein and lactic acid. These quality shifts correlated with microbial and metabolic changes. In the M group, alpha-linolenic acid metabolism was downregulated and Alternaria enriched, while the NPK group had enhanced flavone biosynthesis and a reduced level of Lactiplantibacillus. The B group had enhanced glycine, serine, and threonine metabolism, and displayed the most complex microbial networks along with increased levels of Lactiplantibacillus and Aspergillus. Overall, these results demonstrate that Trichoderma-based fertilization enhances silage quality by promoting accumulation of beneficial microbes and increasing flux through specific metabolic pathways, potentially offering a sustainable strategy for alpine forage improvement.

INSTRUMENT(S): Liquid Chromatography MS -

PROVIDER: MTBLS14092 | MetaboLights | 2026-03-20

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14092_LC-MS___metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14092_LC-MS___metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS14092.txt	Txt

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Project description:succinate (SUC), as a feed additive, can regulate muscle fiber structure and fat deposition, and has a positive effect on meat quality. In this study, 30 male Tan sheep lambs were selected to investigate the effects of SUC in diet on Tan sheep production performance, lamb quality, muscle fiber structure, liver and longissimus' muscle entities (LT) transcriptomes. Different levels of SUC were fed (0%, 1.0%, 2.0%, respectively) and the experiment lasted for 60 days. Compared with CON group, 2% SUC group significantly increased average daily gain from 1 to 15 days and 46 to 60 days during the experiment period, and significantly increased dry matter intake from 1 to 15 days, 16 to 30 days and 46 to 60 days during the experiment period (P<0.05). Both 1% SUC group and 2% SUC group could decrease feed to gain ratio and increase carcass weight (P<0.05). The slaughter rate of 2% SUC group was significantly increased (P=0.012). In both 1% SUC and 2% SUC groups, shear force and cooking loss were decreased, intramuscular fat was increased, and a* of LT muscle was increased (P=0.036). In addition, the muscle fiber structure of Tan sheep LT muscle in 1% SUC group and 2% SUC group was changed, the diameter of muscle fiber was decreased, the cross-sectional area of type Ⅰ and type Ⅱa muscle fiber was decreased, and the density of type Ⅰ muscle fiber was increased (P<0.05). Transcriptomic results showed that the addition of SUC can change the expression of muscle development and muscle fiber type transformation genes. Differential genes are mainly involved in various pathways related to lipid metabolism, energy metabolism and muscle development, improve muscle glucose uptake capacity, and improve meat quality in circadian rhythm. In conclusion, SUC can regulate the nutritional metabolism of Tan Sheep, change the muscle fiber structure of Tan Sheep, and improve the production performance and meat quality. Our results show that adding 1% SUC to the Tan sheep diet is productively more beneficial.

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Trichoderma-based fertilizer enhances quality of Elymus breviaristatus silage via microbial and metabolic modulation

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