Inhibition of jasmonic acid-isoleucine conjugating enzyme JAR1 shifts the local and systemic leaf signals and metabolic profiles in Arabidopsis

ABSTRACT:

• Jasmonates (JAs)-mediated pathways are central signaling hubs in plant defense responses. However, the identification of mobile and non-mobile signals involved in downstream systemic signaling is still less studied.

• Here, we investigate the role of the jasmonic acid-isoleucine conjugating enzyme, JAR1, in shifting wound-induced local and systemic metabolic profiles using LC-MS/MS for untargeted metabolomics, and the mobility of jasmonic acid-isoleucine (JA-Ile) in wound-induced local and systemic defense using LC-MS/MS for targeted jasmonate analysis in Arabidopsis thaliana leaves.

• The use of jarin-1, a specific inhibitor of JA-Ile biosynthesis, suggested that JA-Ile is synthesized de novo in the particular tissues, rather than being a mobile signal. In addition, inhibition of JAR1 enzyme activity affected an array of downstream metabolic pathways, locally and systemically, such as amino acids and carbohydrate metabolism.

• This study suggests that the occurrence and spread of local and systemic downstream signals very likely depends on JAR1 activity, and this enzyme exclusively regulates a series of metabolic pathways under both wounding and non-wounding conditions.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14117 | MetaboLights | 2026-03-31

REPOSITORIES: MetaboLights