Dataset Information

Inhibition of jasmonic acid-isoleucine conjugating enzyme JAR1 shifts the local and systemic leaf signals and metabolic profiles in Arabidopsis

ABSTRACT:

• Jasmonates (JAs)-mediated pathways are central signaling hubs in plant defense responses. However, the identification of mobile and non-mobile signals involved in downstream systemic signaling is still less studied.

• Here, we investigate the role of the jasmonic acid-isoleucine conjugating enzyme, JAR1, in shifting wound-induced local and systemic metabolic profiles using LC-MS/MS for untargeted metabolomics, and the mobility of jasmonic acid-isoleucine (JA-Ile) in wound-induced local and systemic defense using LC-MS/MS for targeted jasmonate analysis in Arabidopsis thaliana leaves.

• The use of jarin-1, a specific inhibitor of JA-Ile biosynthesis, suggested that JA-Ile is synthesized de novo in the particular tissues, rather than being a mobile signal. In addition, inhibition of JAR1 enzyme activity affected an array of downstream metabolic pathways, locally and systemically, such as amino acids and carbohydrate metabolism.

• This study suggests that the occurrence and spread of local and systemic downstream signals very likely depends on JAR1 activity, and this enzyme exclusively regulates a series of metabolic pathways under both wounding and non-wounding conditions.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14117 | MetaboLights | 2026-03-31

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	a_MTBLS14117_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14117_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS14117.txt	Txt

Items per page:

1 - 4 of 4

Similar Datasets

Project description:In the absence of adaptive immunity displayed by animals, plants respond locally to biotic challenge via inducible basal defense networks activated through recognition and response toconserved pathogen associated molecular patterns (PAMPs). In addition, immunity can be induced in tissues remote from infection sites via systemic acquired resistance (SAR), initiated following gene-for-gene recognition between plant resistance proteins and microbial effectors.The nature of the mobile signal and remotely activated networks responsible for establishing SAR remain unclear. Here we show that despite the absence of PAMP contact, systemically responding leaves rapidly activate a SAR transcriptional signature with strong similarity to local basal defense. Jasmonates have previously been implicated in systemic signalling in response to wounding and plant herbivory but not SAR. We present several lines of evidence that suggest jasmonates may also be central to SAR. Jasmonic acid (JA) rapidly accumulates in phloem exudates of leaves challenged with an avirulent strain of Pseudomonas syringae. In systemically responding leaves transcripts associated with jasmonate biosynthesis are upregulated and JA increases transiently. SAR can be mimicked by foliar JA application and is abrogated in mutants impaired in jasmonate synthesis or response. We conclude that, jasmonate signalling appears to mediate long-distance information transmission. Moreover, the systemic transcriptional response shares extraordinary overlap with local herbivory and wounding responses, indicating that jasmonates may be central to an evolutionarily conserved signalling network, which decodes multiple abiotic and biotic stress signals. Experimenter name: William Truman; Experimenter phone: +44 (0)1392 263789; Experimenter fax: +44 (0)1392 263434; Experimenter address: School of Biosciences; Experimenter address: Geoffrey Pope Building; Experimenter address: Stocker Road; Experimenter address: Exeter; Experimenter address: Devon; Experimenter zip/postal_code: EX4 4QD; Experimenter country: UK Experiment Overall Design: 9 samples were used in this experiment

Dataset Information

Inhibition of jasmonic acid-isoleucine conjugating enzyme JAR1 shifts the local and systemic leaf signals and metabolic profiles in Arabidopsis

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets