Dataset Information

Systems toxicology of a tire-wear pollutant: Multi-omics evidence for bioenergetic reprogramming in freshwater clams

ABSTRACT:

The increasing release of tire-derived transformation products into aquatic ecosystems has raised significant environmental concerns, particularly regarding 6PPD-quinone (6PPDQ), a highly toxic compound formed from a widely used tire antioxidant. Despite growing attention, its chronic effects and underlying mechanisms in benthic invertebrates remain poorly understood. This study investigates the systemic impacts of environmentally relevant concentrations of 6PPDQ on the freshwater bivalve Corbicula fluminea using an integrated multi-omics and physiological framework. A 21-day exposure experiment was conducted at concentrations of 4, 8, and 16 ug L-1 alongside a control. Microbial community dynamics were assessed through 16S rRNA and ITS2 sequencing, while host responses were evaluated using RNA sequencing and untargeted LC-MS metabolomics. Co-occurrence network analysis enabled integration of microbiome, transcriptome, and metabolome datasets, revealing key interaction networks and functional pathways. Additionally, oxidative stress biomarkers and histopathological analyses provided evidence of physiological and tissue-level damage. Overall, this study offers a comprehensive systems-level understanding of 6PPDQ toxicity, contributing to improved ecological risk assessment of emerging tire-derived pollutants in freshwater environments.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14142 | MetaboLights | 2026-03-26

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14142_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS14142_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14142_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS14142_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Compared to freshwater ecosystems, the health status of estuarine waters remains little studied despite their importance for many species. They also represent a zone of interest for Human settlements that make them the final sink of pollution in both the water column and sediment. Once in sediments, pollutants could represent a threat to benthic as well as pelagic estuarine species through resuspension events. In the Seine estuary, the copepod Eurytemora affinis has been previously presented as a relevant species to assess resuspended sediment contamination through the use of fitness-related effects at the individual level. The aim of the present study was to use E. affinis copepods to assess estuarine sediment-derived elutriates toxicity using both a molecular (i.e. transcriptomics) and a behavioral approach. Two sites along the Seine estuary were sampled. They were both under anthropic pressures from the industrial-port activities or wastewater treatment plants (i.e. Tancarville) or agricultural pressure from freshwater affluent (i.e. Fatouville). The analysis of sediments used to prepare elutriates reveals that both sites have close contamination profiles. The transcriptomic analysis reveals that exposure to both sites triggers the dysregulation of genes involved in biological function as defense response, immunity, ecdysone pathway or neurotoxicity. This analysis also reveals a higher count of dysregulated genes in the Fatouville site compared to the Tancarville despite their close contamination profile. These results emphasize the sensitivity of this molecular approach to assess environmental matrix toxicity with E. affinis. The analysis of the swimming behavior of E. affinis did not highlight significant effects after both sites elutriate exposure. However, our strategy to assess E. affinis swimming behavior (i.e the combination of the DanioVision observation chamber and the EthoVision analysis software) allows the discrimination of basal swimming behavior in this species. Thus, it represents a promising standardized tool to assess copepods swimming behavior in ecotoxicological studies.

Dataset Information

Systems toxicology of a tire-wear pollutant: Multi-omics evidence for bioenergetic reprogramming in freshwater clams

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