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Metabolomic Profiling of Non-target Metabolites in Streptomyces sp. LZZY-S40

ABSTRACT: This study aimed to characterize the non-targeted metabolite profile of Streptomyces sp. LZZY-S40 to explore its potential for producing bioactive secondary metabolites. The strain LZZY-S40 was cultivated on ISP 3 agar plates at 28°C for 7 days. Spores were harvested and inoculated into 250 mL Erlenmeyer flasks containing 50 mL of seed medium (pH 7.2–7.4) for 48 hours at 28°C with shaking at 250 rpm. The seed culture (8% v/v) was then transferred into fermentation medium and incubated under the same conditions for 7 days. After fermentation, the culture broth was centrifuged at 12,000 rpm for 10 minutes to separate the supernatant from the mycelial biomass. The supernatant was extracted three times with ethyl acetate, and the mycelial pellet was extracted with methanol. Organic extracts were concentrated under reduced pressure at temperatures below 40°C. The combined crude extracts were analyzed by untargeted liquid chromatography–mass spectrometry (LC–MS). Data were acquired in both positive and negative ionization modes across an m/z range of 50-1200, with chromatographic separation achieved using a gradient elution program at a flow rate of 400 μL/min and a column temperature of 40°C.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14181 | MetaboLights | 2026-03-29

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14181_LC-MS_negative_reverse-phase.txt	Txt
	a_MTBLS14181_LC-MS_positive_reverse-phase.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14181_LC-MS_negative_reverse-phase_v2_maf.tsv	Tabular
	m_MTBLS14181_LC-MS_positive_reverse-phase_v2_maf.tsv	Tabular

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