Metabolomic comparison of RA vs healthy monocytes undergoing different ex vivo activation conditions

ABSTRACT:

Rheumatoid arthritis (RA) monocytes are primed for inflammatory activation, but their disease-intrinsic molecular features have not been systematically profiled across omics layers. We aimed to define RA-associated alterations in primary human monocytes using an integrated multi-omics approach under controlled conditions. Peripheral blood CD14+ monocytes from RA patients and matched healthy donors were analyzed in the undifferentiated state (M0) or after differentiation into classically activated M(IFNy+LPS) or alternatively activated M(IL-4) macrophages, with or without acute lipopolysaccharide (LPS) stimulation. Untargeted metabolomics (untargeted LC–MS/MS) revealed a strong disease-driven signature, characterized by decreased nucleotides, redox metabolites and carnitines, while amino acids and urea cycle intermediates were increased. This MetaboLights Study contains untargeted LC-MS/MS data pertaining to the discovery cohort described above. 

INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS14227 | MetaboLights | 2026-10-16

REPOSITORIES: MetaboLights