Metabolomic data related to the alleviation of cardiac ischemia/reperfusion injury by NADPH derived from pentose phosphate pathway

ABSTRACT:

Exercise promotes physiological cardiomyocyte growth and protects against ischemia/reperfusion (I/R) injury in the heart. The molecular mechanism by which exercise benefits cardiac metabolism and function remains largely unknown. Exercise (swimming or treadmill)-induced physiological hypertrophy models were established in mice. Pentose phosphate pathway (PPP) flux was analyzed by 1,2-13C2 glucose stable isotope tracing. In vitro and in vivo manipulations of glucose-6-phosphate dehydrogenase (G6PD) and NADPH were conducted by using lentivirus- and adeno-associated virus-mediated gene transfer, respectively. Myocardial I/R injury model was established in adult mice. Genetically encoded fluorescent indicators (iNap, roGFP, and Hyper) were used to monitor compartmentalized redox and reactive oxygen species (ROS) in neonatal cardiomyocytes during oxygen glucose deprivation/reperfusion (OGD/R). An iNap biosensor-based high-throughput screen was conducted to identify new agents that elevate NADPH levels in cardiomyocytes.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - HILIC, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS14272 | MetaboLights | 2026-05-14

REPOSITORIES: MetaboLights