Tracer-based Metabolomics Technology to Investigate the Role and Mechanism of Fangji Huangqi Tang in Regulating Lipid Metabolism Disorders in MPC-5 Cells

ABSTRACT: Lipid metabolism disorders play a critical role in the progression of nephrotic syndrome (NS). Although the traditional Chinese medicine formula Fangji Huangqi Tang (FHT) has been shown to restore lipid homeostasis in vivo, its specific molecular targets and effects on dynamic lipid metabolism in podocytes remain unclear. To address this, we developed a 13C16-palmitic acid stable isotope tracer lipidomics platform combined with targeted acylcarnitine metabolomics and molecular biology approaches to investigate the regulatory mechanisms of FHT against Adriamycin-induced lipotoxicity in MPC-5 podocytes. Our results demonstrate that FHT protects podocytes by remodeling lipid metabolism__romoting phospholipid synthesis while inhibiting triglyceride accumulation, and modulating fatty acid metabolism through enhanced mitochondrial β-oxidation and suppressed de novo lipogenesis. These changes reversed mitochondrial dysfunction and preserved podocyte structural integrity.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14277 | MetaboLights | 2026-09-20

REPOSITORIES: MetaboLights