Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of oral ketone ester administration in a pMCAO model (KetoSTROKE study)

ABSTRACT:

Rapid, deep, and sustained systemic ketosis was achieved using an orally administered ketone ester (KE: 3.0 mg/g body weight of (R)-3-hydroxybutyl (R)-3-hydroxybutyrate; DeltaG® Ketone Ester, TΔS® Ltd, Oxfordshire, UK) compared with control (0.9% NaCl) prior to permanent middle cerebral artery occlusion (pMCAO; n=5 mice per group). The severity of cerebral ischemia was assessed in vivo by MRI at 60 minutes after arterial occlusion. To investigate the underlying mechanisms, ex vivo metabolomic analysis of brain tissue was performed. The brain was separated into the ischemic hemisphere and the contralateral control hemisphere.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14363 | MetaboLights | 2026-04-24

REPOSITORIES: MetaboLights

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	a_MTBLS14363_LC-MS_negative_reverse-phase.txt	Txt
	a_MTBLS14363_LC-MS_positive_reverse-phase.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14363_LC-MS_negative_reverse-phase_v2_maf.tsv	Tabular
	m_MTBLS14363_LC-MS_positive_reverse-phase_v2_maf.tsv	Tabular

Items per page:

1 - 5 of 6

Similar Datasets

Semaglutide-induced Loss of Skeletal Muscle Mass is Blunted by Co-administration of Ketone Esters

Project description:Building on our recent work demonstrating that ketone ester therapy prevents semaglutide-induced cardiac mass loss in obese mice, we investigated whether ketone therapy could also preserve skeletal muscle mass during semaglutide treatment. To characterize the molecular changes associated with this treatment, we performed RNA-seq transcriptomic analysis of gastrocnemius muscle from obese mice treated with vehicle, semaglutide, or semaglutide + ketone ester. Our analysis identified 5 dominant transcriptomic clusters enriched for pathways related to mitochondrial function, muscle contraction, translation, proteostasis, and extracellular matrix organization. Semaglutide increased expression of genes related to proteostasis and translation, whereas these changes were normalized with ketone ester co-treatment. In addition, semaglutide suppressed several mitochondrial genes, which were restored with ketone ester co-treatment. Furthermore, semaglutide reduced the expression of genes involved in the mitochondrial electron transport chain while increasing the expression of atrophy related genes, both of which were reversed by ketone ester co-treatment. Collectively, these findings suggest that ketone ester co-therapy mitigates semaglutide-induced transcriptomic alterations in skeletal muscle, providing mechanistic insight into how ketone therapy may help preserve muscle function during GLP-1based weight-loss therapy.

2026-05-27 | GSE326952 | GEO

Transcription profiling of mouse forebrain with induced middle cerebral artery occlusion (MCAO) to study permanent focal cerebral ischemia

Project description:profiling gene transcription in a mouse model of permanent focal cerebral ischemia that was induced by middle cerebral artery occlusion (MCAO)

2009-03-07 | E-TABM-655 | biostudies-arrayexpress

Nanostring Glial Profiling Panel of whole brain RNA from CD73ko and control mice subjected to tMCAO

Project description:CD73ko and control mice were subjected to transient middle cerebral artery occlusion (tMCAO) for 50min or subjected to equally long sham surgery. Whole brain RNA was isolated and analyzed with the NanoString nCounter® mouse glial profiling panel.

2022-05-17 | GSE203161 | GEO

mRNA profiles after experimental ischemia

Project description:To analyze gene expression we isolated total mRNA from the core, periinfarct and contralateral cortex of 60 sprague-dawley rats after 24 hours or 3 days of permanent middle cerebral artery occlusion (pMCAo). Also ipsilateral and contralateral cortex was obtained from sham-operated and healthy animals. A total of 44 microarrays were performed with RNA pooled from 3 independent animals. For each experimental condition the total n number is of 12. Microarray analysis of different areas of the rat brain (core, periinfarct and contralateral cortex) after 24h and 3days of permanent ischemia

2012-12-01 | E-GEOD-36010 | biostudies-arrayexpress

Astrocytic calcium waves signal brain injury to neural stem and progenitor cells

Project description:Gene expression patterns in the SVZ, 48 h after an ischemic lesion caused by permanent middle cerebral artery occlusion (MCAO)

2017-01-11 | GSE93376 | GEO

Effect of stroke on the transcriptome of muscularis macrophages in the small intestines

Project description:To investigate the effect of stroke on the trasnscriptome of intestinal muscularis macrophages, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours

2024-03-24 | GSE216968 | GEO

Effect of stroke on the transcriptome of epithelial cells in the small intestines

Project description:To investigate the effect of stroke on the transcriptome of intestinal epithelial cells, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours

2024-03-24 | GSE216581 | GEO

Effect of stroke on the transcriptome of lamina propria macrophages in the small intestines

Project description:To investigate the effect of stroke on the trasnscriptome of intestinal lamina propria macrophages, mice underwent permanent middle cerebral artery occlusion (pMCAO) or sham surgery for 24 hours

2024-03-24 | GSE216971 | GEO

Expression data from P4H-TM deficient mouse cortexes after 24h of pMCAO

Project description:Genetic deletion of transmembrane prolyl-4-hydroxylase (P4H-TM) in mice leads to increased inflammatory microgliosis and neutrophil infiltration in the cortex after permanent midle cerebral artery occlusion (pMCAO)

2020-11-12 | GSE107127 | GEO

Platelet proteomic profiling during active- and sleep-phase ischemic stroke in mice

Project description:This project contains mass spectrometry-based platelet proteomic data generated from mice under active-phase sham, sleep-phase sham, active-phase permanent middle cerebral artery occlusion and sleep-phase permanent middle cerebral artery occlusion conditions. The study was designed to investigate how activity–sleep state and cerebral ischemic challenge reshape the platelet proteome. Comparative proteomic analysis was used to identify sleep-phase- and ischemia-associated changes in platelet functional programs, with particular focus on ribosomal structural proteins, mitochondrial dynamics, mitochondrial respiratory proteins and pathways related to platelet activation and thrombosis.

2026-06-14 | PXD079729 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data