Lipidomic analysis of Drosophila on different sugar feeding regimes +/- trametinib

ABSTRACT:

Consumption of sugar-rich diets adversely affects health and reduces life expectancy through the excessive production and storage of triacylglycerides (TAGs) leading to metabolic dysfunction. Here, using Drosophila melanogaster, we identify the conserved Ras/MAPK signalling pathway as a key mediator of this response. Pharmacological inhibition of Ras/MAPK signalling with the MEK inhibitor, trametinib, was tested for its effect on phospholipid and triacylglycerol content of whole flies extracted by the MTBE method. Reverse phase LC-MSMS analysis was carried out on a Waters SELECT Cyclic IMS instrument operating in DDA mode.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14468 | MetaboLights | 2026-09-02

REPOSITORIES: MetaboLights