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Metabolomic analysis of extracellular metabolites from bacterial strains SL8, SL12 and their co-culture under cadmium stress

ABSTRACT:

In this study, bacterial strains 'SL8', 'SL12', and the cocultured 'SL8+SL12' were cultivated in LB medium with or without 100 mg/L cadmium(II) for 48 hours (six replicates per treatment). After centrifugation, extracellular metabolites in the supernatant were extracted using a methanol acetonitrile (1:1) solution, followed by vortexing, ultrasonic treatment, precipitation at -20°C, and centrifugation. The dried extract was reconstituted, ultrasonicated, and centrifuged again. A 120 µL aliquot was transferred for LC-MS analysis, and pooled QC samples were prepared from 10 µL of each sample.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14477 | MetaboLights | 2026-05-26

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	a_MTBLS14477_LC-MS_negative_reverse-phase.txt	Txt
	a_MTBLS14477_LC-MS_positive_reverse-phase.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14477_LC-MS_negative_reverse-phase_v2_maf.tsv	Tabular
	m_MTBLS14477_LC-MS_positive_reverse-phase_v2_maf.tsv	Tabular

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