Metabolomic analysis of extracellular metabolites from bacterial strains SL8, SL12 and their co-culture under cadmium stress

ABSTRACT:

In this study, bacterial strains 'SL8', 'SL12', and the cocultured 'SL8+SL12' were cultivated in LB medium with or without 100 mg/L cadmium(II) for 48 hours (six replicates per treatment). After centrifugation, extracellular metabolites in the supernatant were extracted using a methanol acetonitrile (1:1) solution, followed by vortexing, ultrasonic treatment, precipitation at -20°C, and centrifugation. The dried extract was reconstituted, ultrasonicated, and centrifuged again. A 120 µL aliquot was transferred for LC-MS analysis, and pooled QC samples were prepared from 10 µL of each sample.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14477 | MetaboLights | 2026-05-26

REPOSITORIES: MetaboLights