Metabolomics analysis revealed the effects of different treatments on the metabolic profile of HaCaT cells

ABSTRACT: To investigate the regulatory effects of the ECM@SF-AGS-CS-LA hydrogel (HC), exosomes (HB), and their combination (HA) on the metabolic profiles of wound tissues in diabetic mice, HaCaT keratinocytes were employed as an in vitro cellular model. Untargeted metabolomics profiling was performed on HaCaT cells subjected to the respective treatments. Five pairwise comparisons were conducted: HC versus HD (diabetic control), HB versus HD, HA versus HD, HA versus HC, and HA versus HB. KEGG pathway enrichment analysis was applied to identify biologically relevant pathways significantly associated with differentially abundant metabolites (P < 0.05). This analysis revealed 8, 28, 30, 30, and 97 significantly enriched pathways in the HC vs HD, HB vs HD, HA vs HD, HA vs HC, and HA vs HB comparisons, respectively.

INSTRUMENT(S): Liquid Chromatography MS - alternating - normal-phase

PROVIDER: MTBLS14481 | MetaboLights | 2026-05-14

REPOSITORIES: MetaboLights