In-situ metabolome of Ramalina farinacea from thalli collected across six European regions during winter and summer

ABSTRACT: Samples of the model lichen Ramalina farinacea were collected from six sites (countries) across a geographical gradient in Europe: Czech Republic, Estonia, Finland, Norway, Spain, and Sweden. Sampling was carried out in winter (January-March 2023) and summer (June-July 2023), with six individuals collected per site and season. Metabolites were extracted and derivatized according to a protocol for plant samples (Lisec et al., 2006), with major modifications. Briefly, methanol extracts from dry samples were obtained by grinding and 70ºC incubation. Extracts were vacuum dried and derivatized by methoxyamination followed by silylation. Derivatized samples were analyzed by GC-MS. GC-MS analysis was performed on an Agilent 5977A mass spectrometer with a quadrupole analyzer and an HP-5MS UI capillary column (30 m × 0.25 mm × 0.25 mm; Agilent 19091S-433UI). Mass spectra were acquired in electron impact mode (70 eV), with a scan range of 30–650 m/z and a solvent delay of 8 min. Each sample was injected in duplicate as technical replicates. Peak deconvolution, spectral matching, and integration were performed using MassHunter GC/MS Acquisition and MassHunter Qualitative software (Agilent Technologies). C7-C30 alkanes were used for peak alignment. Metabolites were annotated by comparing spectra with the NIST commercial library (Ausloos et al., 1999) and manually validated against the Golm Metabolome Database (GMD; (Kopka, 2006)).

INSTRUMENT(S): Gas Chromatography MS - positive - low-polarity

PROVIDER: MTBLS14580 | MetaboLights | 2026-07-22

REPOSITORIES: MetaboLights