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Lipidomic profiling of Scd1- and Scd2-deficient C2C12 cells during brown adipogenic differentiation with oleic acid supplementation

ABSTRACT: This study investigated the lipidomic remodeling of Scd1- and Scd2-deficient C2C12 cells during brown adipogenic differentiation. Scd1 or Scd2 knockout cells and corresponding control cells were induced to undergo brown adipocyte-like differentiation for 96 h. To evaluate whether exogenous monounsaturated fatty acid supplementation could rescue lipid metabolic alterations caused by Scd1 or Scd2 deficiency, cells were supplemented with 10 μM oleic acid during differentiation. Lipid extracts were analyzed by LC-MS-based lipidomics to characterize changes in lipid classes and molecular species associated with Scd1 or Scd2 loss and oleic acid-mediated rescue during the differentiation process.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS14692 | MetaboLights | 2026-06-23

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14692_LC-MS_alternating_reverse-phase.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14692_LC-MS_alternating_reverse-phase_v2_maf.tsv	Tabular
	s_MTBLS14692.txt	Txt

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