Discovery of pseudobaptigenin synthase in fungal-infected Trifolium pratense roots

ABSTRACT:

Isoflavonoids, and their pterocarpan derivatives, are important phytoalexin defense molecules in legumes (Fabaceae). They are commonly associated with antimicrobial activity and thought to be synthesized de novo or accumulated in response to microbial pathogens. Comparative metabolomics of Fusarium oxysporum or Phoma medicaginis-infected red clover roots reveals dynamic regulation of isoflavonoid metabolic flux. Using both a targeted (authentic standards) and untargeted (MS/MS of unknown features) approach, we identified over 40 putative (iso)flavonoid structures, including the highly abundant O-methylated isoflavones (formononetin and biochanin A), many glucosyl- and malonyl-derivatives, and various pterocarpan species.

INSTRUMENT(S): Liquid Chromatography MS -

PROVIDER: MTBLS14741 | MetaboLights | 2026-06-11

REPOSITORIES: MetaboLights