Integrated transcriptome and metabolome analysis reveals the regulatory mechanism underlying fluoride tolerance in XSKD silkworm strain

ABSTRACT: XueSong KD (XSKD) is a fluoride-tolerant Bombyx mori strain that grows normally under long-term fluoride exposure, whereas its molecular tolerance mechanism remains elusive. Herein, we performed combined transcriptomic and untargeted metabolomic profiling using pre-molt fourth-instar XSKD larvae fed either NaF-treated or distilled water-soaked mulberry leaves. In total, we identified 90 differentially accumulated metabolites and 572 differentially expressed genes, with significant expression differences between the two groups. GO and KEGG enrichment revealed that 13-Hydroxy-9Z,11E-octadecadienoic acid (13-(S)-HODE) is the core differential metabolite enriched in the linoleic acid metabolism pathway. Further pathway-associated analysis demonstrated that uncharacterized gene LOC101737302 was significantly downregulated, while the CYP gene CYP338A1 was markedly upregulated, both closely governing downstream 13-(S)-HODE biosynthesis. Collectively, our multi-omics data first uncovered the synergistic regulation between lipid metabolism and xenobiotic detoxification underlying fluoride resistance in XSKD, providing fundamental insights into lipid-mediated immune response against fluoride toxicity and a valuable dataset for future silkworm fluoride-tolerance research.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14966 | MetaboLights | 2026-07-07

REPOSITORIES: MetaboLights