Impacts of fixation processing workflows on the volatile and non-volatile metabolomic profiles of Gougunao green tea

ABSTRACT:

This study compared four fixation processing workflows and their effects on the volatile and non-volatile metabolomic profiles of Gougunao green tea: fully mechanical fixation (M1), mechanical-manual hybrid fixation (M2), manual-mechanical hybrid fixation (M3), and fully manual fixation (M4). An integrated UHPLC-MS/MS and HS-SPME-GC-MS strategy was used to profile non-volatile and volatile metabolites, resulting in 2,105 putatively annotated non-volatile metabolic features and 1,066 volatile metabolites. PCA showed clear workflow-associated separation in both LC-MS and GC-MS datasets, while OPLS-DA supported pairwise discrimination among most comparisons. Differential screening using FDR < 0.05 and |log2FC| > 1 identified 16-183 differential non-volatile metabolites and 0-26 differential volatile metabolites across the six pairwise comparisons, demonstrating that different fixation processing workflows are associated with distinct chemical profiles in Gougunao green tea.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Gas Chromatography MS - positive - low-polarity, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS15065 | MetaboLights | 2026-07-17

REPOSITORIES: MetaboLights