Sample Preparation for Integrated Metabolome and Exposome-Wide Association Studies (MWAS/ExWAS)

ABSTRACT:

Exposome- and metabolome-wide association studies rely on advanced instrumentation, typically anchored in liquid chromatography high-resolution mass spectrometry (LC-HRMS) to investigate exposure-effect associations. Sample preparation for biological fluids is a delicate matter since it is essential to find a pragmatic balance between sensitivity, chemical coverage, robustness, and time efficiency to meet the requirements for large-scale epidemiological studies.

This dataset served as the basis for establishing a protein precipitation workflow in a scalable, high-throughput format for human plasma and urine. Different protocols were evaluated utilizing a xenobiotic mixture containing >200 exposure compounds based on extraction recovery, repeatability, and applicability for non-targeted analysis (NTA). The tested high-throughput options for both matrices included a protein precipitation workflow in 96-well plates, and a phospholipid removal plate. For urine, a dilute-and-shoot approach was additionally tested.

Comparative data obtained from measurements of NIST plasma standard reference material (SRM 1950) is included for the final workflow for benchmarking purposes.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS15100 | MetaboLights | 2026-07-20

REPOSITORIES: MetaboLights