LC-MS/MS Quantification of Nuclear α-KG in NSCLC

ABSTRACT: This study contains targeted LC-MS/MS quantification of α-ketoglutarate (α-KG) in NSCLC cells using 13C-labeled α-KG internal standard. Nuclear α-KG levels were measured in A549, A549/DTX, PC9, and PC9/OR cells. In addition, nuclear α-KG was quantified in GLUD1-overexpressing A549 cells and GLUD1-knockdown A549/DTX cells. Mitochondrial α-KG measurements were performed exclusively in A549/DTX cells. The dataset includes raw SCIEX LC-MS/MS data, sample metadata, and processed quantitative results.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase

PROVIDER: MTBLS15115 | MetaboLights | 2026-07-21

REPOSITORIES: MetaboLights