Multi-omics insights into the effects of Citrus reticulata-derived Bacillus cereus C3X-5 on the fermentation quality of cigar tobacco leaves

ABSTRACT: This study investigates the potential of Bacillus cereus C3X-5, a strain isolated from Citri Reticulatae Pericarpium (CRP), as a bio-enhancer for improving cigar tobacco leaves (CTL) fermentation quality. By integrating physicochemical analysis, metagenomics, and untargeted metabolomics, we systematically evaluated the strain's impact. The results demonstrate that C3X-5 inoculation significantly elevated amylase and protease activities, directed microbial community succession by enriching beneficial genera like Aspergillus, and optimized the metabolic profile. This dual strategy promoted the accumulation of desirable aroma compounds (e.g., pyrazines and ketones) while suppressing green off-notes, thereby enhancing the overall sensory quality of the fermented tobacco.

INSTRUMENT(S): Liquid Chromatography MS - positive - acquity-uplc-hss-t3, Liquid Chromatography MS - negative - acquity-uplc-hss-t3

PROVIDER: MTBLS15119 | MetaboLights | 2026-07-24

REPOSITORIES: MetaboLights