Bile Acid Metabolome in Hepatopancreas Tissue of Common Carp

ABSTRACT:

This study investigated the regulatory effects of three probiotics (P. pentosaceus, B. coagulans, and E. faecalis) on bile acid metabolism in common carp (Cyprinus carpio) fed a high-carbohydrate-high-fat (HCHF) diet for 56 days. A targeted metabolomics approach using UHPLC-QTRAP-MS/MS with scheduled multiple reaction monitoring (MRM) was employed to quantify 10 bile acid components in hepatopancreatic tissues, including free bile acids (CDCA, CA, DCA, LCA, alpha-MCA) and their taurine-conjugated forms (TCDCA, TCA, TDCA, TLCA, Talpha-MCA, Tbeta-MCA, Tomega-MCA). The analysis aimed to elucidate whether probiotic supplementation alleviates HCHF-induced glycolipid metabolic disorders through modulating bile acid profiles and FXR signaling pathway. Furthermore, molecular docking was performed to predict the binding affinities of these bile acids to common carp FXR protein, with CDCA showing the strongest binding energy of -6.859 kcal/mol. The results revealed that all three probiotics significantly increased the contents of FXR-activating free bile acids (CDCA, CA, DCA, LCA) while decreasing conjugated bile acid levels, thereby inhibiting gluconeogenesis and lipogenesis through the FXR-SHP-CYP7A1/CYP8B1 and FXR-SHP-HNF4 alpha/FOXO1 pathways.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase

PROVIDER: MTBLS15295 | MetaboLights | 2026-08-09

REPOSITORIES: MetaboLights