Untargeted metabolomics analysis of intestinal contents in Common Carp

ABSTRACT:

To elucidate the metabolic mechanisms underlying the beneficial effects of Enterococcus faecalis on host health under high-carbohydrate-high-fat (HCHF) stress, we performed an untargeted metabolomics analysis on intestinal contents of common carp (Cyprinus carpio). Fish were fed an HCHF diet with or without E. faecalis supplementation (1 x 10^8 CFU/g) for 56 days. After a 24-h fasting, intestinal contents were collected and analyzed using UHPLC-Q-Orbitrap MS in both positive and negative ion modes. Given that E. faecalis significantly enriched the gut beneficial bacterium Cetobacterium somerae, this study aimed to identify key differential metabolites and affected metabolic pathways, and to explore their correlations with the altered microbiota. The results will help uncover the probiotic-host metabolic crosstalk and provide mechanistic insights for aquaculture applications.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS15296 | MetaboLights | 2026-08-09

REPOSITORIES: MetaboLights