Targeted metabolomic profiling of FH-deficient clear cell renal cell carcinoma cells

ABSTRACT: This study investigated metabolic alterations associated with fumarate hydratase (FH) deficiency in clear cell renal cell carcinoma (ccRCC). Targeted metabolomic analysis was performed in 786-O and RCC4 cells with or without FH knockdown, using three biological replicates per condition. Intracellular metabolites were analyzed by LC-MS/MS using an Agilent 1290 Infinity II UHPLC system coupled to a SCIEX Triple Quad 6500+ mass spectrometer operating in multiple-reaction-monitoring (MRM) mode. The analysis was used to characterize metabolic changes associated with FH deficiency, with particular emphasis on intracellular fumarate accumulation.

INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic, Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS15359 | MetaboLights | 2026-10-16

REPOSITORIES: MetaboLights