Metabolome of the Medicago truncatula symbiosome space, bacteroid, and nodule cytosol

ABSTRACT: This study characterizes the metabolome of the Medicago truncatula-Sinorhizobium meliloti symbiosis. Symbiosome space, bacteroid, and nodule cytosol fractions were isolated from 4-week-old root nodules and profiled by untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polar metabolites were separated on a BEH Amide column and non-polar metabolites on a reversed-phase C18 column, each acquired in positive and negative ionization modes. Three biological replicates were analyzed per sample type, together with pooled quality-control samples. This dataset accompanies our proteomic and metabolomic profiling of the symbiosome and supports the finding that the symbiosome space is an active metabolic compartment enriched in soluble sugars and amino acids.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - positive - reverse-phase, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS15392 | MetaboLights | 2026-08-19

REPOSITORIES: MetaboLights