Dataset Information

Evidence for quorum sensing and differential metabolite production by Ruegeria pomeroyi in response to DMSP

ABSTRACT: Microbial alteration and remineralization of organic matter in the ocean play essential roles in the carbon cycle. The impact of individual compounds as either growth substrates or infochemicals is still poorly constrained in models, particularly for organisms with relatively versatile genomes. Here, we use metabolomics techniques to characterize the response of a heterotrophic marine bacterium, Ruegeria pomeroyi DSS-3, to growth on the abundant algal metabolite dimethylsulfoniopropionate (DMSP). DMSP is a valuable source of reduced carbon and sulfur as well as a chemo-attractant. It is present at higher concentrations during phytoplankton blooms. When cultivated on DMSP, R. pomeroyi synthesized a quorum sensing molecule, N-(3-oxotetradecanoyl)-L-homoserine lactone, which was not synthesized at the same levels during growth on propionate, another carbon substrate. More broadly, we observed differential production of intra- and extracellular metabolites with implications for nitrogen assimilation and microbial cross-feeding. This multi-faceted response suggests that R. pomeroyi uses a two-component regulatory system dependent on the presence of DMSP and high cell densities to activate a suite of metabolic changes that may shape how it accesses nutrients and impacts the surrounding microbial consortium.

OTHER RELATED OMICS DATASETS IN: PRJNA125229

INSTRUMENT(S): TSQ Vantage (Thermo Scientific), Thermo Scientific LTQ FT Ultra

SUBMITTER: Krista Longnecker

PROVIDER: MTBLS157 | MetaboLights | 2015-01-29

REPOSITORIES: MetaboLights

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Publications

Evidence for quorum sensing and differential metabolite production by a marine bacterium in response to DMSP.

Johnson Winifred M WM Kido Soule Melissa C MC Kujawinski Elizabeth B EB

The ISME journal 20160216 9

Microbes, the foundation of the marine foodweb, do not function in isolation, but rather rely on molecular level interactions among species to thrive. Although certain types of interactions between autotrophic and heterotrophic microorganisms have been well documented, the role of specific organic molecules in regulating inter-species relationships and supporting growth are only beginning to be understood. Here, we examine one such interaction by characterizing the metabolic response of a hetero ...[more]

PMID: 26882264

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Dataset Information

Evidence for quorum sensing and differential metabolite production by Ruegeria pomeroyi in response to DMSP

Publications

Evidence for quorum sensing and differential metabolite production by a marine bacterium in response to DMSP.

Similar Datasets

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