Fecal short-chain fatty acids in DSS-induced colitis mice treated with a D-lactate-free probiotic

ABSTRACT: Targeted GC-MS quantification of short- and branched-chain fatty acids in feces of C57BL/6J (B6J-UniBe) mice with acute dextran sulfate sodium (DSS)-induced colitis. Mice received the D-lactate-free probiotic consortium Pro4-50 by daily oral gavage either from the start of the experiment (Pre, n=10) or after the 5-day DSS period (Post, n=9), or no probiotic (DSS, n=7). Feces collected at day 10 were extracted with MTBE and analysed on a TRACE 1610 GC coupled to a TSQ 9610 triple quadrupole mass spectrometer in SIM mode using stable isotope-labelled internal standards and 13-level calibration curves. Acetate, propionate, butyrate, isovalerate, 2-methylbutyrate and valerate are reported in µmol/g feces. This dataset accompanies a companion MetaboLights study of targeted polar fecal metabolomics (LC-MS/MS) in an independent cohort of the same experimental model.

INSTRUMENT(S): Gas Chromatography MS - positive - high-polarity

PROVIDER: MTBLS15952 | MetaboLights | 2026-10-08

REPOSITORIES: MetaboLights