Dataset Information
Non-targeted multimodal metabolomics and lipidomics data from ovine rumen fluid fractions
Ontology highlight
ABSTRACT: From an animal production and health perspective, our understanding of the metabolites in ruminant biofluids, particularly rumen fluid across different host species is poorly understood. Metabolomics is a powerful and sensitive approach for investigating low molecular weight metabolite profiles present in rumen biofluids. It can be used to identify potential roles of metabolites in the rumen microbiome and provide and understanding of host-level regulatory mechanisms associated with animal production. The rumen is a strictly anaerobic environment enriched with a complex community of bacteria, protozoa, fungi, archaea and bacteriophages. Here, we present a metabolomic dataset generated using hydrophilic interaction liquid chromatography (HILIC) and semi-polar (C18) chromatography methods coupled to high resolution mass spectrometry (MS), collected in both positive and negative ionization modes, of ovine rumen samples that were fractionated based on molecular weight (20 kDa, 8-10 kDa and 100 Da). This study highlights the potential of HILIC and C18 chromatography combined with non-targeted mass spectrometric methods to detect the polar and semi-polar metabolite species of the ruminal fluid metabolome.
INSTRUMENT(S): Liquid Chromatography MS -
SUBMITTER: Nikola Palevich
PROVIDER: MTBLS1717 | MetaboLights | 2021-08-23
REPOSITORIES: MetaboLights
Publications
Untargeted Multimodal Metabolomics Investigation of the <i>Haemonchus contortus</i> Exsheathment Secretome.
Cells 20220815 16
In nematodes that invade the gastro-intestinal tract of the ruminant, the process of larval exsheathment marks the transition from the free-living to the parasitic stages of these parasites. To investigate the secretome associated with larval exsheathment, a closed in vitro system that effectively reproduces the two basic components of an anaerobic rumen environment (CO<sub>2</sub> and 39 °C) was developed to trigger exsheathment in one of the most pathogenic and model gastrointestinal parasitic ...[more]
